Enhancement of vascularization and granulation tissue formation by growth factors in human platelet-rich plasma-containing fragmin/protamine microparticles
Enhancement of vascularization and granulation tissue formation by growth factors in human platelet-rich plasma-containing fragmin/protamine microparticles
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DOI：
10.1002/jbm.b.31824
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发表时间：
2011-05-01
影响因子：
3.4
通讯作者：
Kiyosawal, Tomoharu
中科院分区：
文献类型：
--
作者：
Takikawa, Megumi;Nakamura, Shin-Ichiro;Kiyosawal, Tomoharu
本研究的目的是评估人富血小板血浆（PRP）的fragmin/鱼精蛋白微粒（F/P MP）作为蛋白载体对新生血管和肉芽组织形成的影响。冻融PRP含有高浓度的各种生长因子（GF），F/P MP有效地吸附这些GF。人微血管内皮细胞（MVEC）和真皮成纤维细胞（DFC）在含有4%PRP的培养基中最佳生长，并且F/PMP的添加显著维持和保护PRP在37 ℃下孵育超过10天的增殖活性。当PRP含有F/P MP皮下注射到小鼠背部，显着的新血管形成诱导注射部位附近的炎性细胞的过滤增强从第3天到第30天，与对照组相比（注射PRP，F/P MP，和盐水）。含PRP的F/P MP和单独的PRP均诱导注射部位肉芽组织的显著形成。然而，诱导肉芽组织的厚度仅在含PRP的F/P MP注射组中保持良好30天。这些结合的GFs可以在体外和体内从F/P MPs中逐渐扩散和释放。因此，含有PAP的F/P MP在体内比单独的PRP提供显著更高的血管化和纤维组织形成诱导。(C)2011 Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater 97 B：373-380，2011.
The purpose of this study was to evaluate effects of human platelet-rich plasma (PRP)-containing fragmin/protamine microparticles (F/P MPs) as a protein carrier on neovascularization and granulation tissue formation. Frozen and thawed PRP contains high concentrations of various growth factors (GFs) and F/P MPs effectively adsorb those GFs. Human microvascular endothelial cells (MVECs) and dermal fibroblast cells (DFCs) were optimally grown in medium containing 4% PRP and the addition of F/P MPs significantly maintained and protected the proliferative activity of PRP incubated at 37 C for more than 10 days. When PRP-containing F/P MPs were subcutaneously injected into the back of mice, significant neovascularization was induced near the injected site with enhanced filtration of inflammatory cells from day 3 to day 30, compared with controls (injections of PRP, F/P MPs, and saline). Both PRP-containing F/P MPs and PRP alone induced significant formation of granulation tissue at the injected site. However, thickness of induced granulation tissues was well maintained for 30 days only in PRP-containing F/P MP-injected group. Those bound GFs may be gradually diffused and released from F/P MPs in vitro and in vivo. Thereby, PAP-containing F/P MPs offer significantly higher inductions of vascularization and fibrous tissue formation in vivo than PRP alone. (C) 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 97B: 373-380, 2011.