The expressed L1 proteins of HPV-1, HPV-6, and HPV-11 display type-specific epitopes with native conformation and reactivity with neutralizing and nonneutralizing antibodies.
The expressed L1 proteins of HPV-1, HPV-6, and HPV-11 display type-specific epitopes with native conformation and reactivity with neutralizing and nonneutralizing antibodies.
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HPV-1、HPV-6 和 HPV-11 表达的 L1 蛋白显示出具有天然构象的类型特异性表位以及与中和和非中和抗体的反应性。
DOI：
10.1159/000163906
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Jenson,AB
中科院分区：
文献类型：
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作者：
Hines,JF;Ghim,SJ;Christensen,ND;Kreider,JW;Barnes,WA;Schlegel,R;Jenson,AB
先前的研究表明，人乳头瘤病毒（HPV）1型LI蛋白，在COS细胞中表达的SV 40为基础的载体，显示天然病毒粒子的构象表位特征。在这项研究中，我们分析了HPV-1，HPV-6和HPV-11 LI蛋白的表达，以确定重组LI蛋白表达的构象表位的形式。使用免疫荧光和免疫沉淀技术，针对天然HPV-11病毒体产生的多克隆和单克隆抗体（MAb）与HPV-6和/或HPV-11的表达的LI蛋白反应，但不与HPV-1反应。类似地，针对HPV-1病毒体产生的多克隆抗体和单克隆抗体与HPV-1的表达的LI蛋白反应，但不与HPV-6或HPV-11反应。在中和小鼠胎儿包皮异种移植物的HPV-11感染的两种单克隆抗体中，一种与HPV-6和HPV-11的表达的LI蛋白反应，另一种仅与HPV-11反应。非中和构象依赖性单克隆抗体与HPV-6和HPV-11的表达的LI蛋白反应。这些结果表明，表达的HPV LI蛋白保留类型特异性，中和，和非中和构象表位和COS细胞可用于评估宿主对这些表位的免疫应答。
Previous studies demonstrated that the human papillomavirus (HPV) type 1 LI protein, expressed in cos cells by an SV40-based vector, displays conformational epitopes characteristic of native virions. In this study, we analyzed the expression of HPV-1, HPV-6, and HPV-11 LI proteins in order to determine the forms of conformational epitopes expressed by recombinant LI proteins. Using both immunofluorescence and immunoprecipitation techniques, polyclonal and monoclonal antibodies (MAbs) generated against native HPV-11 virions reacted with expressed LI proteins of HPV-6 and/or HPV-11, but not HPV-1. Similarly, polyclonal antibodies and MAbs generated against HPV-1 virions reacted with the expressed LI protein of HPV-1, but not HPV-6 or HPV-11. Of two MAbs that neutralized HPV-11 infection of murine fetal foreskin xenografts, one reacted with the expressed LI protein of both HPV-6 and HPV-11, and the other reacted with HPV-11 only. A nonneutralizing conformationally dependent MAb reacted with the expressed LI protein of both HPV-6 and HPV-11. These results demonstrate that expressed HPV LI proteins retain type-specific, neutralizing, and nonneutralizing conformational epitopes and that cos cells may be utilized to evaluate host immune responses to such epitopes.