Identification of source populations for reintroduction in extinct populations based on genome-wide SNPs and mtDNA sequence: a case study of the endangered subalpine grassland butterfly Aporia hippia (Lepidoptera; Pieridae) in Japan.
Identification of source populations for reintroduction in extinct populations based on genome-wide SNPs and mtDNA sequence: a case study of the endangered subalpine grassland butterfly Aporia hippia (Lepidoptera; Pieridae) in Japan.
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基于全基因组 SNP 和 mtDNA 序列鉴定重新引入灭绝种群的来源种群：以日本濒危亚高山草原蝴蝶 Aporia hippia（鳞翅目；粉蝶科）为例。
DOI：
10.1007/s10841-022-00369-4
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发表时间：
2022
影响因子：
1.9
通讯作者：
T.
中科院分区：
文献类型：
--
作者：
Nakahama;N.;Hanaoka;T.;Itoh;T.;Kishimoto;T;Ohwaki;A.;Matsuo;A.;Kitahara;M.;Usami;S.-I.;Suyama;Y.;Suka;T.
在濒危物种的恢复方案中，使用遗传上密切相关的种群作为再引入来源是至关重要的，因为遗传上遥远的种群可能会导致遗传干扰。Aporia hippia（鳞翅目;粉蝶科）是日本濒临灭绝的亚高山草原蝴蝶，其分布区仅限于本州岛中部的浅间山、八岳山和赤石山。在八岳山脉，其种群可能已经灭绝，目前正在考虑从其他地点重新引入。在此，我们估计的遗传多样性和空间遗传结构。日本的hippiain，以确定候选源种群，通过测序（MIG-seq）和线粒体DNA测序方法，通过多重简单序列重复基因分型在灭绝种群中重新引入。MIG-seq方法表明群体之间存在非常少的遗传差异。线粒体DNA测序方法确定了两种单倍型：单倍型A在浅间，八岳和北方赤石山脉，和单倍型B在中部和南部赤石山脉。这两个单倍型表现出只有一个碱基对之间的替换theme. Implications昆虫保护：根据这些结果，我们建议存在两个保护单位：第一组的人口组成的浅间，八岳，和北方赤石山脉，和第二组的中部和南部赤石山脉。然而，根据这种遗传相似性，所有的A.考虑到近亲繁殖衰退的风险或在不同环境中适应的困难，日本的河马也可以被定义为一个保护单位组。
The use of genetically closely related populations as reintroduction sources is crucial in restoration programmes for endangered species because genetically distant populations may cause genetic disturbances.Aporia hippia(Lepidoptera; Pieridae) is an endangered subalpine grassland butterfly in Japan and its distribution area is restricted to the Asama, Yatsugatake and Akaishi Mountains in central Honshu Island. In the Yatsugatake Mountains, its population is probably already extinct, and reintroduction from the other sites is now under consideration. Here, we estimated the genetic diversity and spatial genetic structure ofA. hippiain Japan to identify candidate source populations for reintroduction in extinct populations via multiplexed inter-simple sequence repeat genotyping by sequencing (MIG-seq) and mitochondrial DNA sequencing methods. The MIG-seq method indicated the presence of very few genetic differences among the populations. The mitochondrial DNA sequencing method identified two haplotypes: haplotype A in the Asama, Yatsugatake and northern Akaishi Mountains, and haplotype B in the central and southern Akaishi Mountains. The two haplotypes exhibited only a one base pair substitution between them.Implications for insect conservation: Based on these results, we suggest the existence of two conservation units: the first group consisting of populations in the Asama, Yatsugatake, and northern Akaishi Mountains, and the second group consisting of those in the central and southern Akaishi Mountains. According to this genetic proximity, however, all populations ofA. hippiain Japan can also be defined as one conservation unit group, given the risk of inbreeding depression or difficulty of adaptation in different environments.