Putative transmembrane transporter modulates higher-level aggression in Drosophila
Putative transmembrane transporter modulates higher-level aggression in Drosophila
复制标题
DOI：
10.1073/pnas.1618354114
复制
发表时间：
2017-02-28
影响因子：
11.1
通讯作者：
Kravitz, Edward A.
中科院分区：
文献类型：
--
作者：
Chowdhury, Budhaditya;Chan, Yick-Bun;Kravitz, Edward A.
通过对35代二元斗争的优胜者的选择，我们产生了一种极具攻击性的欺负蝇系，它几乎总是在与亲本野生型Canton-S种群的斗争中获胜。在发育过程中，Bully表型的维持依赖于温度，当果蝇在19摄氏度下饲养时，表型就会消失。这种差异使我们能够进行RNA-seq实验，并鉴定出在霸凌中差异表达两倍或更大的有限数量的基因；其中一个是推测的跨膜转运蛋白CG13646，它在霸凌中表现出一致和可重复的双重下调。我们通过RNAi方法，用CG13646突变系检测了该基因对表型的因果影响。在所有情况下，CG13646表达减少约一半导致高侵袭性表型，部分类似于在恶霸蝇中看到的表型。该基因是一个非常有趣的溶质载体蛋白（SLCs）家族的成员，其中一些被认为参与哺乳动物系统兴奋性和抑制性神经终端的谷氨酰胺/谷氨酸和GABA代谢循环。
By selection of winners of dyadic fights for 35 generations, we have generated a hyperaggressive Bully line of flies that almost always win fights against the parental wild-type Canton-S stock. Maintenance of the Bully phenotype is temperature dependent during development, with the phenotype lost when flies are reared at 19 degrees C. No similar effect is seen with the parent line. This difference allowed us to carry out RNA-seq experiments and identify a limited number of genes that are differentially expressed by twofold or greater in the Bullies; one of these was a putative transmembrane transporter, CG13646, which showed consistent and reproducible twofold down-regulation in Bullies. We examined the causal effect of this gene on the phenotype with a mutant line for CG13646, and with an RNAi approach. In all cases, reduction in expression of CG13646 by approximately half led to a hyperaggressive phenotype partially resembling that seen in the Bully flies. This gene is a member of a very interesting family of solute carrier proteins (SLCs), some of which have been suggested as being involved in glutamine/glutamate and GABA cycles of metabolism in excitatory and inhibitory nerve terminals in mammalian systems.