Measurement of bone blood flow using the hydrogen washout technique - Part I: Quantitative evaluation of tissue perfusion in the laboratory rat
Measurement of bone blood flow using the hydrogen washout technique - Part I: Quantitative evaluation of tissue perfusion in the laboratory rat
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DOI：
10.1002/jor.20562
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发表时间：
2008-06-01
影响因子：
2.8
通讯作者：
Bishop, Allen T.
中科院分区：
文献类型：
--
作者：
Pelzer, Michael;Larsen, Mikko;Bishop, Allen T.
骨中血流的测量在临床和实验环境中都是令人感兴趣的。这种测定将理想地提供准确、定量和可重复的数据，即使是在实验动物的小骨骼中，也具有相对简单和安全的技术。提供绝对或定量测量的方法包括正电子发射断层扫描，使用放射性或荧光标记微球的"同位素分馏"，以及通过注射或吸入递送的可扩散示踪剂的洗脱速率的测量。在这篇文章中，我们详细描述了原始Whiteside氢冲洗技术的修改，使用现代氢传感器，探针放置的显微操作器和定制软件，大大简化了血流测定，并在实验动物的小骨骼中有效。2008骨科研究学会。出版社：Wiley Periodicals Inc.
The measurement of blood flow in bone is of interest in both clinical and experimental settings. Such determinations would ideally provide accurate, quantitative, and reproducible data with relatively simple and safe technology, even in the small bones of experimental animals. Methods that provide absolute or quantitative measurements include positron emission tomography, "isotope fractioning" using radioactive or fluorescent-labeled microspheres, and measurement of the rate of washout of diffusible tracers delivered either by injection or inhalation. In this article, we describe in detail a modification of the original Whiteside hydrogen washout technique, using modern hydrogen sensors, a micromanipulator for probe placement, and custom software that greatly simplifies blood flow determination and is effective in the small bones of experimental animals. 2008 Orthopaedic Research Society. Published by Wiley Periodicals. Inc.