The age-related decline in interleukin-3 expression in mice.
The age-related decline in interleukin-3 expression in mice.
复制标题
小鼠中白细胞介素 3 表达与年龄相关的下降。
DOI：
10.1016/0024-3205(88)90211-1
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发表时间：
1988
期刊：
影响因子：
6.1
通讯作者：
Cheung,HT
中科院分区：
文献类型：
--
作者：
Li,DD;Chien,YK;Gu,MZ;Richardson,A;Cheung,HT
Spleen lymphocytes form 5− to 37-month-old C57BL/6J mice were stimulated by concanavalin A (con A) in vitro, and the interleukin-3 (IL-3) expression was assessed by measuring the IL-3 activity in culture supernatants and the cytoplasmic IL-3 mRNA levels. The activity and mRNA level of IL-3 was maximum at 20 hr after culturing in the presence of con A. The IL-3 activity in the culture supernatants and the IL-3 mRNA level in lymphocytes declined 70% to 80% between 5 and 37 months of age. Northern blot analysis revealed no change in the size of IL-3 mRNA between young and old mice. When the expression of IL-3 and interleukin-2 (IL-2) by con A-stimulated lymphocytes was compared, both interleukins showed a similar declined with age.
