Immobilization of high-affinity heparin oligosaccharides to radiofrequency plasma-modified polyethylene.
Immobilization of high-affinity heparin oligosaccharides to radiofrequency plasma-modified polyethylene.
复制标题
将高亲和力肝素寡糖固定到射频等离子体改性聚乙烯上。
DOI：
10.1002/jbm.820270614
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Marchant,RE
中科院分区：
文献类型：
--
作者：
Yuan,S;Szakalas-Gratzl,G;Ziats,NP;Jacobsen,DW;Kottke-Marchant,K;Marchant,RE
对抗凝血酶 III (ATIII) 具有高亲和力的肝素寡糖已固定在表面改性的 NHLBI 主要参考低密度聚乙烯 (PE) 上。 PE 通过源自 N-乙烯基-2-吡咯烷酮 (PPNVP) 或烯丙醇 (PPAA) 的射频等离子体聚合（厚度<150 nm）薄膜进行改性，并通过化学衍生化与 3-氨基丙基三乙氧基硅烷或氨基封端的聚环氧乙烷偶联。高亲和力肝素寡糖（HA-肝素，抗因子 Xa 活性为 592 ± 120 IU/mg）通过商业粗肝素的部分脱氨裂解制备并通过琼脂糖-ATIII 亲和层析进行分级，通过还原胺化将其固定到表面改性的 PE 上。通过抑制 Xa 因子的显色测定测定，抗凝血活性估计为 30-70 mIU/cm2，结合力估计为 56-119 ng/cm2。通过带有 PEO 间隔基的 PPNVP 修饰固定到 PE 上的 HA-肝素获得了最高的活性。通过金标双抗体法和 SEM 成像，目视确认 ATIII 与固定化 HA-肝素的结合。 © 1993 约翰威利父子公司。
Oligosaccharides of heparin with high affinity for antithrombin III (ATIII) have been immobilized onto surface‐modified NHLBI Primary Reference low density polyethylene (PE). PE was modified by radiofrequency plasma polymerized (<150 nm thick) films derived from N‐vinyl‐2‐pyrrolidone (PPNVP) or allyl alcohol (PPAA), and coupled by chemical derivatization to either 3‐aminopropyltriethoxysilane or amino‐terminated poly ethylene oxide). High affinity heparin oligosaccharides (HA‐heparin, anti‐factor Xa activity of 592 ± 120 IU/mg) prepared by partial deaminative cleavage of commercial crude heparin and fractionated by agarose‐ATIII affinity chromatography, were immobilized to surface‐modified PE by reductive amination. The anticoagulant activity, as determined by a chromogenic assay for the inhibition of factor Xa, was estimated to be 30–70 mIU/cm2, with binding estimated to be 56–119 ng/cm2. The highest activity was obtained for the HA‐heparin immobilized to PE modified by PPNVP with a PEO spacer. Visual confirmation of ATIII binding to immobilized HA‐heparin was demonstrated by a goldlabeled double antibody method with imaging by SEM. © 1993 John Wiley & Sons, Inc.