Oxidative stress in cell culture: an under-appreciated problem?
Oxidative stress in cell culture: an under-appreciated problem?
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DOI：
10.1016/s0014-5793(03)00235-7
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发表时间：
2003-04-10
期刊：
影响因子：
3.5
通讯作者：
Halliwell, B
中科院分区：
文献类型：
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作者：
Halliwell, B
细胞培养研究已经提供了许多关于代谢和信号转导机制以及基因表达、增殖、衰老和死亡的调节的有价值的信息。然而，培养中的细胞可能在许多方面与体内细胞不同。其中之一是细胞培养对细胞施加氧化应激状态。我认为，在培养中存活和生长的细胞可能使用ROS依赖的信号转导途径，很少或从来没有在体内运行。另一个问题是细胞培养基可以催化添加到其中的化合物的氧化，导致明显的细胞效应，其实际上是由于氧化产物如ROS。这些人工制品可能影响了许多关于抗坏血酸、硫醇、类黄酮和其他多酚化合物对培养细胞影响的研究。(C)2003年欧洲生物化学学会联合会。由Elsevier Science B.V.出版，版权所有。
Cell culture studies have given much valuable information about mechanisms of metabolism and signal transduction and of regulation of gene expression, proliferation, senescence, and death. However, cells in culture may behave differently from cells in vivo in many ways. One of these is that cell culture imposes a state of oxidative stress on cells. I argue that cells that survive and grow in culture might use ROS-dependent signal transduction pathways that rarely or never operate in vivo. A further problem is that cell culture media can catalyse the oxidation of compounds added to them, resulting in apparent cellular effects that are in fact due to oxidation products such as ROS. Such artefacts may have affected many studies on the effects of ascorbate, thiols, flavonoids and other polyphenolic compounds on cells in culture. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.