Quadruplex formation by a guanine-rich PNA oligomer
Quadruplex formation by a guanine-rich PNA oligomer
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DOI：
10.1021/ja0446202
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发表时间：
2005-03-30
影响因子：
15
通讯作者：
Armitage, BA
中科院分区：
文献类型：
--
作者：
Datta, B;Bier, ME;Armitage, BA
具有序列 H-G(4)T(4)G(4)-Lys-NH2 (G(4)-PNA) 的富含鸟嘌呤的 PNA 十二聚体与同源序列的 DNA 十二聚体杂交形成四链四链体（Datta, B.；Schmitt, C.；Armitage, B. A. J. Am. Chem. Soc. 2003, 125, 4111-4118）。该报告描述了仅由 PNA 形成的四链体。紫外熔解曲线和荧光共振能量转移实验揭示了鸟嘌呤四分体稳定的多链结构的形成。这些结构的离子依赖性与报道的 DNA 四链体类似。电喷雾电离质谱表明二聚体和四聚体四链体均由G(4)-PNA形成，优选二聚体形式。这些结果对于使用富含 G 的 PNA 与染色体 DNA 中富含 G 的靶标进行同源杂交具有重要意义，并提出了在脂质双层环境中组装四链体结构的其他应用。
A guanine-rich PNA dodecamer having the sequence H-G(4)T(4)G(4)-Lys-NH2 (G(4)-PNA) hybridizes with a DNA dodecamer of homologous sequence to form a four-stranded quadruplex (Datta, B.; Schmitt, C.; Armitage, B. A. J. Am. Chem. Soc. 2003, 125, 4111-4118). This report describes quadruplex formation by the PNA alone. UV melting curves and fluorescence resonance energy transfer experiments reveal formation of a multistrancled structure stabilized by guanine tetrads. The ion dependency of these structures is analogous to that reported for DNA quadruplexes. Electrospray ionization mass spectrometry indicates that both dimeric and tetrameric quadruplexes are formed by G(4)-PNA, with the dimeric form being preferred. These results have implications for the use of G-rich PNA for homologous hybridization to G-rich targets in chromosomal DNA and suggest additional applications in assembling quadruplex structures within lipid bilayer environments.