THE PURIFICATION AND CHROMATOGRAPHY OF BACTERIOPHAGES ON ANION-EXCHANGE CELLULOSE
THE PURIFICATION AND CHROMATOGRAPHY OF BACTERIOPHAGES ON ANION-EXCHANGE CELLULOSE
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DOI：
10.1016/0042-6822(57)90057-0
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发表时间：
1957-01-01
期刊：
影响因子：
3.7
通讯作者：
TAUSSIG, A
中科院分区：
文献类型：
--
作者：
CREASER, EH;TAUSSIG, A
描述了一种从大肠杆菌裂解物中纯化噬菌体T1、T2 r和T2 r+的方法。将裂解物透析并用ECTEOLA阴离子交换吸附剂处理，然后用浓度递增的NaCl溶液处理交换剂。在0.20 [image]NaCl下洗脱病毒，并可通过在20，000 g下离心浓缩，获得的病毒产率为裂解物中存在的病毒产率的25-82%。在T2 r和T2 r+的情况下，噬菌体制剂含有3.3 ± 0.3 × 10-10 [mu]g DNA或蛋白质/感染中心，在T1的情况下，含有1.2 × 10-10 [mu]g DNA和2.4 × 10-10 [mu]g蛋白质/感染中心。在制剂中未检测到非病毒核酸。描述了活病毒和裂解病毒在ECTEOLA柱上的色谱。
A method is described for the purification of bacteriophages T1, T2r and T2r+ from lysates of Escherichia coli. The lysates are dialyzed and treated with ECTEOLA anion exchange adsorbent after which the exchanger is treated with increasing concentrations of NaCl solution. The virus is eluted at 0.20 [image]NaCl and can be concentrated by centrifugation at 20,000 g, yields of virus varying from 25-82% of that present in the lysates being obtained. The bacteriophage preparations contain 3.3 + 0.3 x 10-10 [mu]g Dna or protein/ infective center in the case of T2r and T2r+ and 1.2 x 10-10 [mu]g DNA and 2.4 x 10-10 [mu]g protein/infective center in the case of T1 No non-viral nucleic acid can be detected in the preparations. The chromatography of live and split virus on ECTEOLA columns is described.