Postharvest ASM dipping and DPI pre-treatment regulated reactive oxygen species metabolism in muskmelon (Cucumis melo L.) fruit
Postharvest ASM dipping and DPI pre-treatment regulated reactive oxygen species metabolism in muskmelon (Cucumis melo L.) fruit
复制标题
采后 ASM 浸渍和 DPI 预处理调节甜瓜 (Cucumis melo L.) 果实的活性氧代谢
DOI：
10.1016/j.postharvbio.2014.09.001
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发表时间：
2015-01-01
影响因子：
7
通讯作者：
Dong, Boyu
中科院分区：
文献类型：
--
作者：
Ge, Yonghong;Deng, Huiwen;Dong, Boyu
由粉红色单端孢菌（Trichothecium roseum）引起的粉腐病是甜瓜采后最重要的病害之一。本研究旨在评价甜瓜（Cucumis梅洛L. cv.）用100 mg/L的硫代水杨酸（ASM）和50 μ M的NADPH氧化酶特异性抑制剂二苯碘鎓（DPI）处理后，对愈伤组织的生长有明显的影响。结果表明，接种T. 100 mg/L ASM浸泡可显著降低玫瑰花的生长量（P < 0.05）。H_2O_2的积累、超氧阴离子（O-2（-））的释放、NADPH氧化酶（NOX）、超氧化物歧化酶（SOD）、抗坏血酸过氧化物酶（APX）活性的增强和过氧化氢酶（CAT）活性的抑制均与病斑发育的减缓有关。ASM处理还诱导了抗氧化剂含量，包括抗坏血酸（MA）和还原型谷胱甘肽（GSH）。与ASM处理的果实相比，在ASM处理之前用DPI处理的果实表现出更大的病斑直径。DPI处理抑制了ASM诱导的H2 O2和O-2（-）积累，抑制了NO X、SOD、APX活性的升高，抑制了抗坏血酸（阿萨）和还原型谷胱甘肽（GSH）含量的升高。细胞化学研究表明，H_2O_2和O-2（-）主要沉积在细胞间隙和细胞壁中。这些结果表明，DPI预处理阻止了ASM诱导的ROS积累，导致严重的疾病症状，突出了ROS在ASM诱导的甜瓜果实抗性中的重要作用。(C)2014爱思唯尔有限公司版权所有。
Pink rot caused by Trichothecium roseum is one of the most important postharvest diseases of muskmelon. The present study was to evaluate how disease resistance in muskmelon fruit (Cucumis melo L.cv. Yujingxiang) was affected by dipping with 100 mg/L acibenzolar-S-methyl (ASM) and 50 mu M diphenylene iodonium (DPI), a NADPH oxidase specific inhibitor. Lesion diameters on the fruit inoculated with T. roseum were significantly decreased (P < 0.05) by dipping with 100 mg/L ASM. Decreased lesion development was associated with the accumulation of H2O2, release of superoxide anion (O-2(-)) , enhancement activities of NADPH oxidase (NOX), superoxide dismutase (SOD), ascorbate peroxidase (APX), and inhibition of catalase (CAT) activity. Antioxidant content including ascorbic acid (MA) and reduced glutathione (GSH) was also induced by ASM treatment. Compared with ASM treated fruit, fruit treated with DPI prior to ASM treatment exhibited larger lesion diameter. Moreover, DPI treatment inhibited ASM-induced H2O2 and O-2(-) accumulation, the increase of NOX, SOD, APX activities and content of ascorbic acid (AsA), and reduced glutathione (GSH). Cytochemical studies indicated that H2O2 and O-2(-) were mainly deposited in the intercellular space and cell walls. These results suggest that pre-treatment with DPI prevented accumulation of ROS induced by ASM and resulted in serious disease symptoms, highlighting the important role of ROS in ASM-induced resistance in muskmelon fruit. (C) 2014 Elsevier B.V. All rights reserved.