Pulsed radiofrequency applied to dorsal root ganglia causes a selective increase in ATF3 in small neurons
Pulsed radiofrequency applied to dorsal root ganglia causes a selective increase in ATF3 in small neurons
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DOI：
10.1016/j.ejpain.2005.03.001
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发表时间：
2006-02-01
影响因子：
3.6
通讯作者：
Hall, S
中科院分区：
文献类型：
--
作者：
Hamann, W;Abou-Sherif, S;Hall, S
背景资料：这是一项“概念验证研究”，旨在检验脉冲射频（PRF）在初级传入水平产生细胞应激而无明显热损伤迹象的假设。我们假设细胞应激会导致正常功能受损，并使用转录激活因子3（ATF 3）的表达作为细胞“应激”的指标。（20 ms的500 kHz RF脉冲，以2 Hz的速率输送;最高温度42 ℃）递送到成年大鼠大腿中部的坐骨神经，或到椎间孔远端的L4前主支。对照组为假手术组或L4轴突切断组。术后14天检查所有组织。结果：脊髓切断后，L4背根节神经元胞体无论大小，其ATF 3表达均上调。它也显著上调（p
Background: This is a "proof of concept study" to test the hypothesis that pulsed radiofrequency, PRF, produces cell stress at the primary afferent level without signs of overt thermal damage. We assumed that cell stress would result in impairment of normal function, and used the expression of activating transcription factor 3, ATF3, as an indicator of cellular "stress".Methods: PRF (20 ms of 500-kHz RF Pulses, delivered at a rate of 2 Hz; maximum temperature 42 degrees C) was delivered either to the sciatic nerve of adult rats in mid thigh, or to the L4 anterior primary ramus just distal to the intervertebral foramen. Controls were sham-operated or L4 axotomised. All tissues were examined 14 days after Surgery. The percentage of CGRP- or ATF3-positive DRG neuronal somata was calculated using image analysis software (SigmaScan Pro 4).Results: ATF3 expression was upregulated in L4 DRG neuronal cell bodies, irrespective of their size, after axotomy. It was also upregulated significantly (p