Changes in the glycosylation pattern during embryonic development of mouse kidney as revealed with lectin conjugates
Changes in the glycosylation pattern during embryonic development of mouse kidney as revealed with lectin conjugates
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DOI：
10.1177/35.1.3794309
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发表时间：
1987-01-01
影响因子：
3.2
通讯作者：
Saxen, Lauri
中科院分区：
文献类型：
--
作者：
Laitinen, Liisa;Virtanen, Ismo;Saxen, Lauri
用荧光染料和过氧化物酶偶联凝集素研究了成年和发育中小鼠肾脏凝集素结合位点的分布。还比较了固定方法对凝集素结合模式的影响。未诱导的间充质细胞和输尿管芽的早期后肾与刀豆球蛋白A，Leas culinaris，蓖麻我，和小麦胚芽凝集素反应，而大豆和花生（PNA）凝集素的结合位点只在输尿管芽组织中看到。在诱导，PNA阳性迅速出现在诱导，冷凝领域的后肾间充质。早期肾小球表达不均匀的终端半乳糖基和N-乙酰半乳糖胺基团的足细胞。后来，这些位点消失了，显然被唾液酸覆盖。内皮细胞在成熟过程中也表现出相当的末端糖部分的唾液酸化。许多上述凝集素的结合位点也被发现在发展中的近端和远端小管。终端岩藻糖残基，成熟的近端小管的特点，出现在第13天的发展。双花扁豆凝集素反应，通常见于收集管，出现了第13天。Griffonia simplicifolia-I-B4 isolectin反应仅局限于成人肾皮质的内皮细胞，但在胚胎肾与集合管和足细胞的反应也被发现。这些结果表明，细胞糖复合物在成年小鼠肾脏中的区室化表达是在发育过程中以顺序的方式获得的。这种成熟糖基化模式的连续出现可能反映了肾单位的功能成熟。
Distribution of lectin-binding sites in adult and developing mouse kidney was studied with fluorochrome- and peroxidase-coupled lectins. Effects of fixation methods on lectin-binding patterns were also compared. Un-induced mesenchymal cells and ureter bud of the early metanephros reacted with Concanavalin A, Leas culinaris, Ricinus communis I, and wheat germ agglutinins, whereas binding sites for both soybean and peanut (PNA) agglutinins were seen only in ureter bud tissue. On induction, PNA positivity rapidly appeared in the induced, condensed areas of the metanephrogenic mesenchyme. Early glomeruli expressed heterogeneously terminal galactosyl and N-acetylgalactosaminyl moieties in the podocytes. Later, these sites disappeared and were apparently covered by sialic acids. Endothelia also displayed a comparable sialylation of terminal saccharide moieties during maturation. Binding sites for many of the above lectins were also found in the developing proximal and distal tubules. Terminal fucosyl residues, characteristic of mature proximal tubules, appeared during day 13 of development. Dolichos biflorus agglutinin reactivity, typically seen in the collecting ducts, appeared by day 13. Griffonia simplicifolia-I-B4 isolectin reactivity was exclusively localized to endothelial in adult kidney cortex, but in embryonic kidneys reactivity with collecting duct and podocytes was also seen. These results suggest that the compartmentalized expression of cell glycoconjugates in adult mouse kidney is acquired in a sequential manner during development. Such sequential appearance of the mature glycosylation pattern probably reflects functional maturation of the nephron.