Reparative dentin formation in rat molars after direct pulp capping with growth factors
Reparative dentin formation in rat molars after direct pulp capping with growth factors
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DOI：
10.1016/s0099-2399(98)80166-0
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发表时间：
1998-11-01
影响因子：
4.2
通讯作者：
Tatum, NB
中科院分区：
文献类型：
--
作者：
Hu, CC;Zhang, CH;Tatum, NB
当作为直接盖髓药物应用时，参与正常伤口愈合的生长因子可促进组织修复。在大鼠磨牙中进行最小的牙髓暴露，放置盖髓药物，并密封牙腔。表皮生长因子、碱性成纤维细胞生长因子、胰岛素样生长因子II、血小板衍生生长因子-BB和转化生长因子-β（1）（TGF-β（1））-各自被吸收到无菌胶原膜（BioMend; Calcitek，卡尔斯巴德，CA）上，分别用作牙髓药物。Dycal、未浸渍的胶原膜和无药物用作对照。在手术后2周和3周，从每个治疗方案中采集8份样本。对牙髓软硬组织反应进行分级。数据采用单因素方差分析和Tukey-Kramer检验进行分析。2周后无显著差异。与手术对照组相比，用TGF-β（1）处理的牙髓在第3周显示出软组织和硬组织愈合的显著改善。我们的结论是，TGF-β（1）作为盖髓药物促进大鼠磨牙修复性牙本质的形成。
Growth factors involved in normal wound healing may promote tissue repair when applied as a direct pulp capping medication. A minimal pulp exposure was made in rat molars, a pulp capping medication was placed, and the cavity was sealed. Epidermal growth factor, basic fibroblast growth factor, insulin-like growth factor II, platelet-derived growth factor-BB, and transforming growth factor-beta(1) (TGF-beta(1))-each absorbed onto a sterile collagen membrane (BioMend; Calcitek, Carlsbad, CA)were used separately as pulpal medicaments. Dycal, unimpregnated collagen membrane, and no medication were used as controls. Eight samples from each treatment regimen were collected 2 and 3 weeks after surgery. Pulpal soft and hard tissue responses were graded. Data were analyzed by one-way ANOVA and Tukey-Kramer tests. No significant differences were detected after 2 wk. Pulp treated with TGF-beta(1) showed significantly improved soft and hard tissue healing at week 3, compared with the procedure control. We conclude that TGF-beta(1) as a pulp-capping medication enhances reparative dentin formation in rat molars.