Steinernema litorale n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Japan
Steinernema litorale n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Japan
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滨海斯氏线虫 n.
DOI：
10.1163/1568541044038650
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发表时间：
2004
期刊：
影响因子：
1.2
通讯作者：
M. Yoshida
中科院分区：
文献类型：
--
作者：
M. Yoshida
从日本本州岛爱知县Atsumi半岛伊拉戈角沙质土壤中分离到一种新的昆虫病原线虫——litorale Steinernema n. sp。新种的形态特征为：体长909±42.1 (834-988)μm，头至排泄孔距离61±3.3 (54-69)μm，尾长83±4.5 (72-91)μm，比值E值0.73(0.68-0.83)，侧场有9条明显的纹路；雄尾两代均有短柄，针尖形（中等弓形，较重，针尖柄较长），第一代雄尾的针尖长度为75±4.8 (67-89)μm，管柄长度为53±4.0 (44-64)μm；两代雌性外阴均为双翅型外阴，尾尖，第一代雌性的比值D值为45.2（33.3 ~ 56.8）。利用RFLP图谱将新种与形态相似的S. feltiae、S. thanhi、S. karii、S. scarabaei、S. kraussei、S. oregonense、S. loci和S. diaprepesi进行了区分。形态学和分子生物学的研究表明，在表型上litorale n. sp.与S. feltiae最相似。然而，杂交试验表明，这两个物种是生殖隔离的。
A new entomopathogenic nematode, Steinernema litorale n. sp., was isolated from sandy soils in Cape Irago, Atsumi Peninsula, Aichi, Honshu Island, Japan. The new species is characterised by the following morphological characters: infective third-stage juvenile with a body length of 909 ± 42.1 (834–988) μm, distance from head to excretory pore of 61 ± 3.3 (54–69) μm, tail length of 83 ± 4.5 (72–91) μm, ratio E value of 0.73 (0.68–0.83) and lateral field with nine distinct lines; male tail with a mucron in both generations, spicule shape (moderately arcuate, heavy, spicule with elongated manubrium), spicule length of 75 ± 4.8 (67–89) μm and gubernaculum length of 53 ± 4.0 (44–64) μm in the first generation male; female vulva with a double-flapped epiptygma and a tail with a pointed tip in both generations, and ratio D value of 45.2 (33.3–56.8) in the first generation female. The new species was distinguished from the morphologically similar species, S. feltiae, S. thanhi, S. karii, S. scarabaei, S. kraussei, S. oregonense, S. loci and S. diaprepesi by RFLP profiles. Morphological and molecular studies indicated that S. litorale n. sp. was phenotypically most similar to S. feltiae. However, cross hybridisation tests demonstrated that the two species were reproductively isolated.