Nuclear organization and gene expression.
Nuclear organization and gene expression.
复制标题
核组织和基因表达。
DOI：
10.1006/excr.1996.0358
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发表时间：
1996
影响因子：
3.7
通讯作者：
Spector,DL
中科院分区：
文献类型：
--
作者：
Spector,DL
在活跃转录的细胞中，参与前 mRNA 剪接的因子在荧光显微镜水平上以斑点模式定位。斑点图案对应于电子显微镜水平上的染色质间颗粒簇和染色质周围原纤维。根据[3H]尿苷掺入研究，转录被认为发生在染色质周围原纤维处，而不是发生在染色质间颗粒簇内。我们已经证明，在抑制 RNA 聚合酶 II 转录或前 mRNA 剪接时，剪接因子会重新分布并优先定位于染色质间颗粒簇，这些颗粒簇变得更大且形状更均匀。将外源 DNA 模板引入细胞核会导致剪接因子募集到新的转录位点。这些数据表明剪接因子位于储存和/或重组位点（染色质间颗粒簇），并被招募到转录活性位点（染色质周围原纤维）。此外，这些数据表明前mRNA剪接因子的斑点定位反映了细胞的转录和前mRNA剪接活性。
In actively transcribing cells, factors involved in pre-mRNA splicing localize in a speckled pattern at the fluorescence microscopic level. The speckled pattern corresponds to interchromatin granule clusters and perichromatin fibrils at the electron microscopic level. Based upon [3H]uridine incorporation studies transcription is thought to occur at the perichromatin fibrils and not within interchromatin granule clusters. We have shown that upon inhibition of RNA polymerase II transcription or pre-mRNA splicing, splicing factors redistribute and preferentially localize to interchromatin granule clusters, which become larger and more uniform in shape. Introduction of exogenous DNA templates into the cell nucleus results in a recruitment of splicing factors to the new sites of transcription. These data suggest that splicing factors are localized at storage and/or reassembly sites (interchromatin granule clusters) and are recruited to active sites of transcription (perichromatin fibrils). Furthermore, these data demonstrate that the speckled localization of pre-mRNA splicing factors is a reflection of the transcriptional and pre-mRNA splicing activities of the cell.