ORGANIZATION OF OVINE CORTICOTROPIN-RELEASING FACTOR IMMUNOREACTIVE CELLS AND FIBERS IN THE RAT-BRAIN - AN IMMUNOHISTOCHEMICAL STUDY
ORGANIZATION OF OVINE CORTICOTROPIN-RELEASING FACTOR IMMUNOREACTIVE CELLS AND FIBERS IN THE RAT-BRAIN - AN IMMUNOHISTOCHEMICAL STUDY
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DOI：
10.1159/000123454
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发表时间：
1983-01-01
影响因子：
4.1
通讯作者：
VALE, WW
中科院分区：
文献类型：
--
作者：
SWANSON, LW;SAWCHENKO, PE;VALE, WW
在正常成年大鼠的脑中，并在脑室内注射秋水仙碱，或已被肾上腺切除灌注前3-60天的动物的脑中，促肾上腺皮质激素释放因子（CRF）的免疫反应细胞和纤维的分布进行了检查。CRF免疫反应性位于至少3个功能不同的系统中。首先，正中隆起的神经血管区中的大多数CRF染色的纤维，其推测调节ACTH和β-肾上腺素的释放。来自垂体的内啡肽似乎出现在下丘脑的室旁核（PVH）中。约2000个CRF染色的细胞分布在PVH的所有8个部分中，尽管大多数（80%）细胞集中在小细胞分裂中，而较少数量（约10%）的细胞集中在小细胞分裂中。15%）被发现在大细胞分裂的催产素能细胞占主导地位的部分。这似乎是大脑中受肾上腺切除术影响（染色强度增加）的唯一CRF染色途径。第二，在基底端脑、下丘脑和脑干中的一系列细胞群，已知在自主反应的介导中起作用，这些细胞群包含CRF染色的神经元。这些区域通过内侧前脑束和脑室周围系统中的染色纤维相互连接，包括杏仁核中央核、无名质、终纹床核、内侧和外侧视前区、外侧下丘脑区、中央灰质、被盖背外侧核、蓝斑、臂旁核、迷走神经背侧复合体、以及含有A1和A5儿茶酚胺细胞群的区域。第三，在大脑皮层的大部分区域都发现了散在的CRF染色细胞。大多数这样的细胞局限于新皮层的第二层和第三层，它们的双极形状表明它们是中间神经元。这些细胞最常见于边缘系统区域，包括前额区、扣带回和与鼻裂接壤的区域。在齿状回和阿蒙角的背侧部也发现散在的免疫反应细胞。PVH显然在ACTH和β-肾上腺素的调节中起关键作用。脑垂体释放内啡肽脑中含有CRF的通路可能参与自主反应的介导。
The distribution of corticotropin-releasing factor (CRF)-immunoreactive cells and fibers was examined in the brains of normal adult rats, and in the brains of animals that had been pretreated with intraventricular injections of colchicine, or had been adrenalectomized 3-60 days before perfusion. CRF immunoreactivity is localized in at least 3 functionally distinct systems. First, most of the CRF-stained fibers in the neurohemal zone of the median eminence, which presumably modulate the release of ACTH and .beta.-endorphin from the pituitary, appear to arise in the paraventricular nucleus of the hypothalamus (PVH). About 2000 CRF-stained cells are distributed throughout all 8 parts of the PVH, although a majority (80%) of the cells are concentrated in the parvocellular division, and a smaller number (.apprx. 15%) are found in parts of the magnocellular division in which oxytocinergic cells predominate. This appears to be the only CRF-stained pathway in the brain that is affected (increased staining intensity) by adrenalectomy. Second, a series of cell groups in the basal telencephalon, hypothalamus, and brain stem that are known to play a role in the mediation of autonomic responses contain CRF-stained neurons. These areas, which are interconnected by stained fibers in the medial forebrain bundle and the periventricular system, include the central nucleus of the amygdala, substantia innominata, bed nucleus of the stria terminalis, medial and lateral preoptic areas, lateral hypothalamic area, central gray, laterodorsal tegmental nucleus, locus ceruleus, parabrachial nucleus, dorsal vagal complex, and regions containing the A1 and A5 catecholamine cell groups. Third, scattered CRF-stained cells are found throughout most areas of the cerebral cortex. Most such cells are confined to layers II and III in the neocortex, and their bipolar shape suggests that they are interneurons. These cells are most common in limbic regions including prefrontal areas, the cingulate gyrus, and areas bordering the rhinal fissure. Scattered immunoreactive cells are also found in dorsal parts of the dentate gyrus and Ammon''s horn. The PVH apparently plays a critical role in the modulation of ACTH and .beta.-endorphin release from the pituitary. CRF-containing pathways in the brain may be involved in the mediation of autonomic responses.