Increased binding of Sp1 to the gamma-globin gene promoter upon site-specific cytosine methylation.
Increased binding of Sp1 to the gamma-globin gene promoter upon site-specific cytosine methylation.
复制标题
位点特异性胞嘧啶甲基化后 Sp1 与 γ-珠蛋白基因启动子的结合增加。
DOI：
10.1002/ajh.2830460302
复制
发表时间：
1994
影响因子：
12.8
通讯作者：
DeSimone,J
中科院分区：
文献类型：
--
作者：
Sengupta,PK;Lavelle,D;DeSimone,J
We have studied the effect of site‐specific cytosine methylation on the binding of nuclear proteins to the γ‐globin promoter region from −71 to −34. This sequence was recently shown to contain a stage selector element responsible for increased transcription from the γ compared to the β promoter when both are linked to Locus Control Region enhancer sequences. We observed that Sp1 binding to this sequence is increased upon site‐specific cytosine methylation, such that only methylation of the cytosine in the −50 CpG dinucleotide effects Sp1 binding. Methylation of the distal C of the Hpall site has no effect. The possible functional role of Sp1 as a repressor of γ‐globin synthesis is discussed. © 1994 Wiley‐Liss, Inc.
