DEPOSITION OF PLASMA FIBRONECTIN IN TISSUES
DEPOSITION OF PLASMA FIBRONECTIN IN TISSUES
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DOI：
10.1073/pnas.78.5.3218
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
RUOSLAHTI, E
中科院分区：
文献类型：
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作者：
OH, E;PIERSCHBACHER, M;RUOSLAHTI, E
采用免疫荧光法研究小鼠静脉注射人纤维连接蛋白的组织分布。在注射人纤维连接蛋白的小鼠组织中检测到人纤维连接蛋白。注射后纤维连接蛋白的分布与小鼠纤维连接蛋白无明显差异。在制备组织切片之前，注射的小鼠肝脏中灌注的人纤维连接蛋白染色不受影响，而作为对照的小鼠注射的人IgG在组织中未被免疫荧光检测到。小鼠注射纤维连接蛋白片段，分子量接近20万。这些片段缺乏纤维连接蛋白多肽的部分NH2和COOH末端，包括具有链间二硫键的部分，但保留了与胶原蛋白和肝素结合的能力，以及介导细胞附着的能力。结果表明，纤维连接蛋白分子的完整性对于纤维连接蛋白在组织中的积累非常重要。纤维连接蛋白注射入组织表明循环纤维连接蛋白有助于组织的细胞外基质。
Tissue distribution of human fibronectin injected i.v. into mice was studied by using immunofluorescence with species-specific antisera to human and mouse fibronectins. Human fibronectin was detected in the tissues of mice injected with human fibronectin. The distribution of the injected fibronectin was indistinguishable from that of mouse fibronectin. The staining for human fibronectin in the livers of injected mice was unaffected by perfusion of the livers prior to the preparation of tissue sections, and human IgG injected in mice as a control was not detectable in tissues by immunofluorescence. Mice were injected with fragments of fibronectin with MW close to 200,000. These fragments lack parts of the NH2 and COOH termini of the fibronectin polypeptide, including the part with the interchain disulfide bond(s), but retain the ability to bind to collagen and heparin and the ability to mediate cell attachment. They showed essentially no tissue incorporation, suggesting that the integrity of the fibronectin molecule is important for the accumulation of fibronectin in tissues. The incorporation of injected fibronectin into tissues suggests that circulating fibronectin contributes to the extracellular matrix of tissues.