Comparison of pinoresinol diglucoside production by Phomopsis sp. XP-8 in different media and the characterisation and product profiles of the cultivation in mung bean.
Comparison of pinoresinol diglucoside production by Phomopsis sp. XP-8 in different media and the characterisation and product profiles of the cultivation in mung bean.
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DOI：
10.1002/jsfa.7593
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发表时间：
2016-09
影响因子：
4.1
通讯作者：
Yan Zhang;Junling Shi;Zhenhong Gao;J. Che;D. Shao;Yanlin Liu
中科院分区：
文献类型：
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作者：
Yan Zhang;Junling Shi;Zhenhong Gao;J. Che;D. Shao;Yanlin Liu
背景拟茎点霉XP-8是一株能在体外产生半夏醇二葡萄糖苷（PDG）的内生真菌，在植物以外的PDG生物合成中具有潜在的应用价值。为了提高拟茎点霉XP-8的PDG产量，对18种不同的天然材料进行了固态培养试验。结果大多数供试天然物质对PDG的产生有促进作用。来自绿豆的补充剂产生最高的PDG产量和比其他材料更好的真菌生长。此外，在绿豆上发酵后，获得了松醇单葡萄糖苷、松醇和其他底物（苯丙氨酸、对香豆酸、肉桂酸、咖啡酸和阿魏酸）。此外，PDG生产高得多时，绿豆纳入固态琼脂与液体培养基。在含有100 g kg（-1）葡萄糖的绿豆颗粒培养基上使用拟茎点霉XP-8的固态培养物在9天内获得最高的松醇二葡萄糖苷产量（在新鲜培养物中为72.1 mg kg（-1））。绿豆水溶性多糖被鉴定为拟茎点霉XP-8产生PDG的主要促进剂。结论绿豆尤其是其水溶性多糖组分是促进拟茎点霉XP-8生产PDG的有效天然物质。© 2015化学工业协会。
BACKGROUND Phomopsis sp. XP-8 is an endophytic fungus with the ability to produce pinoresinol diglucoside (PDG) in vitro and thus has potential application in biosynthesis of PDG independent of plants. In order to enhance the production of PDG, 18 different natural materials were tested in solid-state cultivation of Phomopsis sp. XP-8. RESULTS Most of the tested natural materials promoted the production of PDG. A supplement derived from mung beans produced the highest PDG yield and better fungal growth than the other materials. Also, pinoresinol monoglucoside, pinoresinol and other substrates (phenylalanine, p-coumaric acid, cinnamic acid, caffeic acid, and ferulic acid) were obtained after fermentation on mung beans. Furthermore, PDG production was much higher when mung beans were incorporated into solid state agar versus a liquid medium. The highest pinoresinol diglucoside production (72.1 mg kg(-1) in fresh culture) was obtained in 9 days using a solid state culture of Phomopsis sp. XP-8 on a mung bean grain medium containing 100 g kg(-1) glucose. Mung bean water-soluble polysaccharide was identified as a major promoter of PDG production by Phomopsis sp. XP-8. CONCLUSION Mung bean, especially its water-soluble polysaccharide fraction, was an efficient natural material to promote PDG production by Phomopsis sp. XP-8. © 2015 Society of Chemical Industry.