Molecular properties of rod and cone visual pigments from purified chicken cone pigments to mouse rhodopsin in situ
Molecular properties of rod and cone visual pigments from purified chicken cone pigments to mouse rhodopsin in situ
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DOI：
10.1039/b416731g
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发表时间：
2005-01-01
影响因子：
3.1
通讯作者：
Shichida, Y
中科院分区：
文献类型：
--
作者：
Imai, H;Kuwayama, S;Shichida, Y
我们研究了视杆色素和视锥色素的分子特性，以阐明视杆色素和视锥色素感光细胞之间光响应的分子机制差异。我们发现，与杆状色素视紫红质相比，锥状色素具有更快的色素再生和更快的间位ii和间位iii中间体衰变。诱变实验表明，在122位和189位的氨基酸残基是这些差异的决定因素。为了研究视色素的分子特性与视杆光感受器的生理之间的关系，我们使用小鼠视紫红质作为模型色素，因为通过基因靶向，色素的光谱特性可以直接与细胞的生理相关。本文综述了视锥色素的光谱特性，并描述了我们利用高性能电荷耦合器件（CCD）分光光度计对小鼠视紫红质的研究。
We have investigated the molecular properties of rod and cone visual pigments to elucidate the differences in the molecular mechanism(s) of the photoresponses between rod and cone photoreceptor cells. We have found that the cone pigments exhibit a faster pigment regeneration and faster decay of meta-II and meta-III intermediates than the rod pigment, rhodopsin. Mutagenesis experiments have revealed that the amino acid residues at positions 122 and 189 in the opsins are the determinants for these differences. In order to study the relationship between the molecular properties of visual pigments and the physiology of rod photoreceptors, we used mouse rhodopsin as a model pigment because, by gene-targeting, the spectral properties of the pigment can be directly correlated to the physiology of the cells. In the present paper, we summarize the spectroscopic properties of cone pigments and describe our studies with mouse rhodopsin utilizing a high performance charge coupled device (CCD) spectrophotometer.