Levels and patterns of genetic variation in Japanese whiting (Sillago japonica) based on mitochondrial DNA control region
Levels and patterns of genetic variation in Japanese whiting (Sillago japonica) based on mitochondrial DNA control region
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基于线粒体 DNA 控制区的日本牙鳕 (Sillago japonica) 遗传变异水平和模式
DOI：
10.1080/24701394.2018.1467411
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发表时间：
2019-01-02
影响因子：
--
通讯作者：
Xiao, Yong-Shuang
中科院分区：
文献类型：
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作者：
Gao, Tian-Xiang;Yang, Tian-Yan;Xiao, Yong-Shuang
日本鳕（Sillagojaponica）是分布于东亚沿着的一种重要经济鱼类，近年来成为一种水产养殖品种。尽管它的商业重要性，种群遗传变异的水平和模式仍然知之甚少。本研究以中国、朝鲜和日本沿海沃茨的14个沿着地点的346份标本为材料，利用线粒体DNA控制区（D-loop）序列分析了它们的遗传变异。共检测到131个多态位点，确定了294个单倍型。在检测范围内检测到具有高水平的单倍型多样性（h = 0.999 +/- 0.001）和核苷酸多样性（l = 0.030 +/- 0.015）的模式。分子方差分析（AMOVA）和F-st分析表明，除伊势湾（IBP）样品与其他样品间的遗传分化外，中国、韩国和日本的群体间均不存在明显的遗传分化。最小生成树（MST）、中性检验、错配分布和贝叶斯天际线分析表明，中国、朝鲜和日本沿海沿着物种在晚更新世冰期和冰消期经历了起源于106-423千年的种群扩张。
Japanese whiting (Sillago japonica) is a commercially important demersal fish distributed along the coasts of East Asia and becomes recently an aquaculture species. Despite its commercial importance, the levels and patterns of population genetic variation remain poorly understood. In this study, 346 specimens were collected from 14 localities along the coastal waters of China, Korea and Japan and their genetic variation was analyzed with mtDNA control region (D-loop) sequences. A total of 131 polymorphic sites were detected which determined 294 haplotypes. A pattern with high levels of haplotype diversity (h = 0.999 +/- 0.001) and nucleotide diversity (л = 0.030 +/- 0.015) was detected in the examined range. Analyses of analysis of molecular variance (AMOVA) and F-st showed that no significant genetic differentiation existed among China, Korea and Japan populations, excepting for the populations between Ise Bay (IBP) sample and the other ones. Minimum spanning tree (MST), neutrality tests, mismatch distribution and Bayesian skyline analyses indicated that the species along coastline of China, Korea and Japan have experienced population expansions originated in its most recent history at about 106-423 kya during the late Pleistocene glaciations and deglaciations periods.