Enhanced Hemocompatibility of Silver Nanoparticles Using the Photocatalytic Properties of Titanium Dioxide.
Enhanced Hemocompatibility of Silver Nanoparticles Using the Photocatalytic Properties of Titanium Dioxide.
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DOI：
10.3389/fbioe.2022.855471
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发表时间：
2022
影响因子：
5.7
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Chen X;Dai S;Liu L;Liu P;Ye P;Liao Y;Zhao A;Yang P;Huang N;Chen J
银纳米粒子（AgNPs）由于其优异的抗菌性能而被广泛应用。然而，较差的血液相容性限制了AgNPs在血液接触材料中的应用。提高含银纳米粒子表面的血液相容性的一般方法是构建屏障层或共粘附的抗凝血生物分子。但这种修饰策略通常准备起来很麻烦，而且应用范围有限。因此，本研究提出了一种简单的紫外光功能化策略，以提高AgNPs的血液相容性。我们将AgNPs负载到二氧化钛（TiO 2）纳米颗粒上形成复合纳米颗粒（Ag@TiO2NPs）。然后，对Ag @ TiO 2 NPs进行UV处理，利用TiO 2纳米颗粒的可扩散光诱导抗凝血性质来增强AgNPs的血液相容性。在沉积到PU表面上之后，光官能化的Ag @ TiO 2 NPs涂层对革兰氏阳性/革兰氏阴性细菌均显示出优异的抗菌性能。此外，体外和离体实验表明，光功能化Ag@TiO2纳米粒子涂层具有良好的血液相容性。该修饰策略为改善金属纳米粒子的血液相容性提供了一种新的解决思路。
Silver nanoparticles (AgNPs) are widely used because of their excellent antimicrobial properties. However, the poor hemocompatibility limits the application of AgNPs in blood contact materials. General approaches to improve the hemocompatibility of AgNPs-containing surfaces are to construct barrier layers or co-immobilize anticoagulant biomolecules. But such modification strategies are often cumbersome to prepare and have limited applications. Therefore, this study proposes a simple UV-photo-functionalization strategy to improve the hemocompatibility of AgNPs. We loaded AgNPs onto titanium dioxide (TiO2) nanoparticles to form a composite nanoparticles (Ag@TiO2NPs). Then, UV treatment was performed to the Ag@TiO2NPs, utilizing the diffusible photo-induced anticoagulant properties of TiO2 nanoparticles to enhance the hemocompatibility of AgNPs. After being deposited onto the PU surface, the photo-functionalized Ag@TiO2NPs coating showed excellent antibacterial properties against both Gram-positive/Gram-negative bacteria. Besides, In vitro and ex-vivo experiments demonstrated that the photo-functionalized Ag@TiO2NPs coating had desirable hemocompatibility. This modification strategy can provide a new solution idea to improve the hemocompatibility of metal nanoparticles.