Soy-dairy protein blend and whey protein ingestion after resistance exercise increases amino acid transport and transporter expression in human skeletal muscle
Soy-dairy protein blend and whey protein ingestion after resistance exercise increases amino acid transport and transporter expression in human skeletal muscle
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DOI：
10.1152/japplphysiol.01093.2013
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发表时间：
2014-06-01
影响因子：
3.3
通讯作者：
Rasmussen, B. B.
中科院分区：
文献类型：
--
作者：
Reidy, P. T.;Walker, D. K.;Rasmussen, B. B.
在休息时或运动后增加氨基酸的可用性（通过输注或摄入）可增强氨基酸向人体骨骼肌的转运。目前尚不清楚是否改变氨基酸的可用性，从摄入不同的膳食蛋白质，可以提高氨基酸转运速率和氨基酸转运蛋白（AAT）mRNA的表达。我们假设，长期高氨基酸血症从ingredients混合蛋白质具有不同的消化率postexercise将增强氨基酸转运到肌肉和AAT表达相比，摄入的快速消化的蛋白质。在一项双盲随机临床试验中，我们研究了16名年轻人在休息和急性阻力运动后，加上运动后（1小时）摄入（大豆-乳制品）蛋白质混合物或乳清蛋白。苯丙氨酸净平衡和运输速率到骨骼肌使用稳定同位素方法结合股动静脉采血和肌肉活检在休息和3和5小时postexercise获得。两组中苯丙氨酸转运入肌肉和选择性AAT [系统L氨基酸转运蛋白1/溶质连接载体（SLC）7A 5、CD 98/SLC 3A 2、系统A氨基酸转运蛋白2/SLC 38 A2、质子辅助氨基酸转运蛋白1/SLC 36 A1、阳离子氨基酸转运蛋白1/SLC 7A 1]的mRNA表达增加的程度相似（P < 0.05）。然而，与乳清蛋白相比，混合蛋白的摄入导致运动后恢复期间延长的正净苯丙氨酸平衡（P < 0.05）。运动后肌原纤维蛋白合成增加组之间相似。我们的结论是，虽然这两种蛋白质来源增强运动后AAT的表达，运输到肌肉中，和肌原纤维蛋白质的合成，运动后摄入的蛋白质混合物的结果在一个稍微延长的净氨基酸平衡的腿相比，乳清蛋白。
Increasing amino acid availability (via infusion or ingestion) at rest or postexercise enhances amino acid transport into human skeletal muscle. It is unknown whether alterations in amino acid availability, from ingesting different dietary proteins, can enhance amino acid transport rates and amino acid transporter (AAT) mRNA expression. We hypothesized that the prolonged hyperaminoacidemia from ingesting a blend of proteins with different digestion rates postexercise would enhance amino acid transport into muscle and AAT expression compared with the ingestion of a rapidly digested protein. In a double-blind, randomized clinical trial, we studied 16 young adults at rest and after acute resistance exercise coupled with postexercise (1 h) ingestion of either a (soy-dairy) protein blend or whey protein. Phenylalanine net balance and transport rate into skeletal muscle were measured using stable isotopic methods in combination with femoral arteriovenous blood sampling and muscle biopsies obtained at rest and 3 and 5 h postexercise. Phenylalanine transport into muscle and mRNA expression of select AATs [system L amino acid transporter 1/solute-linked carrier (SLC) 7A5, CD98/SLC3A2, system A amino acid transporter 2/SLC38A2, proton-assisted amino acid transporter 1/SLC36A1, cationic amino acid transporter 1/SLC7A1] increased to a similar extent in both groups (P < 0.05). However, the ingestion of the protein blend resulted in a prolonged and positive net phenylalanine balance during postexercise recovery compared with whey protein (P < 0.05). Postexercise myofibrillar protein synthesis increased similarly between groups. We conclude that, while both protein sources enhanced postexercise AAT expression, transport into muscle, and myofibrillar protein synthesis, postexercise ingestion of a protein blend results in a slightly prolonged net amino acid balance across the leg compared with whey protein.