Determination of the Synthetic Antioxidants Butylated Hydroxyanisole (BHA) and Butylated Hydroxytoluene (BHT) by Matrix Acidity-Induced Switchable Hydrophilicity Solvent-Based Homogeneous Liquid-Liquid Microextraction (MAI-SHS-HLLME) and High-Performance
Determination of the Synthetic Antioxidants Butylated Hydroxyanisole (BHA) and Butylated Hydroxytoluene (BHT) by Matrix Acidity-Induced Switchable Hydrophilicity Solvent-Based Homogeneous Liquid-Liquid Microextraction (MAI-SHS-HLLME) and High-Performance
复制标题
DOI：
10.1080/00032719.2021.1941072
复制
发表时间：
2021-06
影响因子：
2
通讯作者：
E. Yıldız;H. Çabuk
中科院分区：
文献类型：
--
作者：
E. Yıldız;H. Çabuk
建立了一种快速、简便的样品前处理方法--基质酸度诱导可切换亲水性溶剂均相液-液微萃取，用于从食醋样品中分离合成抗氧化剂丁基羟基苯甲醚和丁基羟基甲苯。以二(2-乙基己基)磷酸(DEHPA)为萃取剂，在碱性条件下，加入食醋，制得亲水性的DEHPA微滴。在这一步不需要离心，因为含有分析物的DEHPA相是通过磁性回收从得到的混合物中收集的，因为它对Fe3O4纳米粒子具有很高的亲和力。解吸后的分析物经磁分离后，用高效液相色谱-紫外检测法进行分析。在优化的条件下，方法的检出限为3.2~5.5µg L−1，线性范围为10~5 0 0μg L−1，精密度良好，相对标准偏差小于7.8%。添加苹果、葡萄和白醋的回收率为86%-105%。
Abstract A rapid and easy sample pretreatment procedure, matrix acidity-induced switchable hydrophilicity solvent-based homogeneous liquid-liquid microextraction, has been developed for the isolation of the synthetic antioxidants butylated hydroxyanisole and butylated hydroxytoluene from vinegar samples. Di-(2-ethylhexyl)phosphoric acid (DEHPA) was selected as the extraction solvent and its hydrophilic form was obtained in alkaline solution followed by the addition of vinegar, which acted as both the sample and the inductor of the switching reaction leading to the formation of DEHPA microdroplets. There was no need to centrifuge at this step, since the DEHPA phase containing the analytes was collected from the resulting mixture through magnetic retrieval due to its high affinity for Fe3O4 nanoparticles. After being magnetically separated, the desorbed analytes were analyzed by high performance liquid chromatography with ultraviolet detection (HPLC-UV). Under the optimized conditions, the limits of detection for the analytes were between 3.2 and 5.5 µg L−1 and the linear dynamic range was from 10 to 500 μg L−1. Good precision was obtained with relative standard deviations less than 7.8%. Recovery assays for spiked apple, grape, and white vinegar provided recoveries from 86 to 105%.