Coronin Function Is Required for Chemotaxis and Phagocytosis in Human Neutrophils1
Coronin Function Is Required for Chemotaxis and Phagocytosis in Human Neutrophils1
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DOI：
10.4049/jimmunol.178.9.5769
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发表时间：
2007-05
期刊：
影响因子：
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通讯作者：
Ming Yan;C. di Ciano-Oliveira;S. Grinstein;W. Trimble
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文献类型：
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作者：
Ming Yan;C. di Ciano-Oliveira;S. Grinstein;W. Trimble
冠蛋白是一类保守的肌动蛋白相关蛋白，参与了许多依赖于肌动蛋白重排的细胞过程。在这项研究中，我们表明，在原代人类中性粒细胞，冠状蛋白-1-4和-7的表达。冠状蛋白-1聚集在迁移的中性粒细胞的前缘和新生的吞噬体。通过转导显性负性形式的蛋白质抑制冠蛋白功能导致抑制趋化性和减少中性粒细胞扩散和粘附。这种抑制似乎与细胞内F-肌动蛋白结构分布的变化相关。此外，吞噬作用受到抑制，但NADPH氧化酶的分泌和活化似乎都不受影响。总之，这些结果表明，冠蛋白所需的肌动蛋白依赖的细胞形态的变化，导致迁移和吞噬作用。
Coronins are a family of conserved actin-associated proteins that have been implicated in a variety of cellular processes dependent on actin rearrangements. In this study, we show that in primary human neutrophils, coronins-1–4 and -7 are expressed. Coronin-1 accumulates at the leading edge of migrating neutrophils and at the nascent phagosome. Inhibition of coronin function by transduction of a dominant-negative form of the protein leads to inhibition of chemotaxis and a reduction in neutrophil spreading and adhesion. This inhibition appears to correlate with changes in the distribution of F-actin structures within the cell. In addition, phagocytosis is inhibited, but neither secretion nor activation of the NADPH oxidase appears to be affected. Together, these results show that coronins are required for actin-dependent changes in cell morphology that lead to migration and phagocytosis.