Caspase-mediated changes in Sir2α during apoptosis
Caspase-mediated changes in Sir2α during apoptosis
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DOI：
10.1016/j.febslet.2006.09.051
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发表时间：
2006-10-30
期刊：
影响因子：
3.5
通讯作者：
Miura, Masayuki
中科院分区：
文献类型：
--
作者：
Ohsawa, Shizue;Miura, Masayuki
沉默信息调节因子2（Silent Information Regulator 2，Sir 2）是一种依赖于NAD（+）的组蛋白脱乙酰酶，它能建立抑制性染色质状态并延长芽殖酵母和线虫的寿命。越来越多的证据表明，其哺乳动物同系物Sir 2 α保护细胞免受应激诱导的凋亡。我们在这里报告，哺乳动物Sir 2 α直接裂解的启动子和执行者半胱天冬酶，并重新定位从细胞核到细胞质中的凋亡细胞。Sir 2 α的这些改变在很大程度上被胱天蛋白酶-9显性负突变体或Bcl-xL抑制。我们的研究结果表明，Sir 2 α在细胞凋亡过程中以caspase依赖的方式发生动态变化。(c)2006年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
Silent information regulator 2 (Sir2) is an NAD(+)-dependent histone deacetylase that establishes repressive chromatin status and extends the life span of both budding yeast and the nematode worm Caenorhabditis elegans. There is growing evidence that its mammalian homologue Sir2 alpha protects cells from stress-induced apoptosis. We report here that mammalian Sir2 alpha was directly cleaved by both initiator and executioner caspases, and relocated from the nucleus to the cytoplasm in apoptotic cells. These alterations of Sir2 alpha were largely inhibited by a caspase-9 dominant-negative mutant or Bcl-xL. Our results indicate that Sir2 alpha undergoes dynamic changes in caspase-dependent manner during apoptosis. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.