Quantitative assessment of mast cells and expression of IgE protein and mRNA for IgE and interleukin 4 in the gastrointestinal tract of healthy dogs and dogs with inflammatory bowel disease.
Quantitative assessment of mast cells and expression of IgE protein and mRNA for IgE and interleukin 4 in the gastrointestinal tract of healthy dogs and dogs with inflammatory bowel disease.
复制标题
定量评估健康犬和患有炎症性肠病的犬胃肠道中肥大细胞以及 IgE 蛋白和 IgE 和白细胞介素 4 mRNA 的表达。
DOI：
10.2460/ajvr.2001.62.211
复制
发表时间：
2001
影响因子：
1
通讯作者：
M. Griot
中科院分区：
文献类型：
--
作者：
C. Locher;A. Tipold;M. Welle;A. Busato;A. Zurbriggen;M. Griot
目的
研究炎症性肠病（IBD）犬的黏膜IgE网络特征，并与健康犬进行比较。
动物
9只健康犬和20只IBD犬。
程序
对犬胃肠道和淋巴结组织切片进行IgE和白细胞介素4（IL-4）特异性mRNA的原位杂交和IgE蛋白和肥大细胞2种标志物（类胰蛋白酶和糜蛋白酶）的免疫组织化学分析。
结果
与健康狗相比，患有IBD的狗的胃肠道粘膜中的IgE蛋白阳性细胞和肥大细胞明显更多。尽管IgE阳性细胞数量显著增加，但在GI粘膜中很少检测到IgE mRNA阳性细胞;大多数IgE mRNA阳性细胞见于肠系膜淋巴结。IL-4 mRNA的信号模式与IgE mRNA相似。
结论和临床相关性
IBD犬中IgE和肥大细胞阳性细胞数量增加提示超敏反应，例如对肠腔中细菌或饮食来源抗原的超敏反应。未来的研究需要阐明这是炎症的原因还是IBD炎症过程的结果。
OBJECTIVE
To characterize the mucosal IgE network in dogs affected with inflammatory bowel disease (IBD) and compare it with that for healthy dogs.
ANIMALS
9 healthy dogs and 20 dogs with IBD.
PROCEDURE
In situ hybridization of mRNA specific for IgE and interleukin 4 (IL-4) and immunohistochemical analysis for IgE protein and 2 markers of mast cells (ie, tryptase and chymase) were performed on tissue sections obtained from the gastrointestinal (GI) tract and lymph nodes of dogs.
RESULTS
Dogs with IBD had significantly more cells positive for IgE protein and more mast cells in the GI mucosa than healthy dogs. Despite this significant increase in number of cells positive for IgE, cells positive for IgE mRNA were rarely detected in the GI mucosa; most cells positive for IgE mRNA were found in mesenteric lymph nodes. Signal pattern of IL-4 mRNA was similar to that of IgE mRNA.
CONCLUSIONS AND CLINICAL RELEVANCE
The increased numbers of cells positive for IgE and mast cells in dogs with IBD suggest hypersensitivity such as hypersensitivity to bacterial or dietary-derived antigens in the intestinal lumen. Future studies need to elucidate whether this represents a cause of inflammation or is a result of the inflammatory process of IBD.