Membrane inlet for mass spectrometric measurement of nitric oxide.
Membrane inlet for mass spectrometric measurement of nitric oxide.
复制标题
用于一氧化氮质谱测量的膜入口。
DOI：
10.1016/j.freeradbiomed.2007.07.026
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发表时间：
2007
影响因子：
7.4
通讯作者：
Silverman,DavidN
中科院分区：
文献类型：
--
作者：
Tu,Chingkuang;Swenson,ErikR;Silverman,DavidN
指出了膜入口质谱法测定水溶液中一氧化氮的优点。膜入口探针是一段1.0 cm的硅橡胶管，连接到通往离子源的真空入口。硅橡胶是一种半渗透性的硅橡胶，允许包括一氧化氮（NO）在内的不带电物质的通量。使用这种入口来测量NO有几个优点，我们在本报告中演示。它提供了一个直接的，连续的，定量测定溶解的一氧化氮浓度在长时间的实时。在我们的系统中使用这样的入口，响应时间为5到7秒，检测下限为1.0 nM。该装置用于测量亚硝酸盐、NONOates和硝普苷溶液中NO的生成。讨论了这种入口在生理系统中测量NO的有用性。
We point out the advantages of membrane inlet mass spectrometry for the measurement of nitric oxide in aqueous solution. The membrane inlet probe was a 1.0-cm segment of Silastic tubing attached to the vacuum inlet leading to the ion source. Silastic is a semipermeable silicon rubber that allows flux of uncharged substances including nitric oxide (NO). The use of such an inlet to measure NO has several advantages that we demonstrate in this report. It provides a direct, continuous, and quantitative determination of dissolved nitric oxide concentrations over long periods of real time. The use of such an inlet in our system had a response time of 5 to 7 s and a detection lower limit with the current model of 1.0 nM. This apparatus was used to measure the generation of NO from solutions of nitrite, NONOates, and nitroprusside. The usefulness of such an inlet in measuring NO in physiological systems is discussed.