Gut microbial composition changes in bladder cancer patients: A case-control study in Harbin, China
Gut microbial composition changes in bladder cancer patients: A case-control study in Harbin, China
复制标题
膀胱癌患者肠道微生物组成的变化：中国哈尔滨的病例对照研究
DOI：
10.6133/apjcn.202007_29(2).0022
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发表时间：
2020-07-01
影响因子：
1.3
通讯作者：
Shan, Yujuan
中科院分区：
文献类型：
--
作者：
He, Canxia;Li, Baolong;Shan, Yujuan
背景与目的：探讨膀胱癌患者肠道菌群的变化。方法和研究设计：招募新诊断的膀胱癌患者。所有参与者都完成了一份关于个人行为和饮食的问卷。通过Illumina HiSeq 2000对从人粪便中提取的总基因组DNA进行焦磷酸测序。通过实时定量PCR测定细菌靶DNA的拷贝数。粪便中短链脂肪酸含量采用气相色谱法测定。采用酶联免疫吸附法测定血清中脂多糖和D-乳酸的浓度。结果：水果摄入量明显低于健康对照组。膀胱癌患者的梭菌群XI和普雷沃菌数量减少。区域细菌和普雷沃氏菌的数量与水果摄入量呈显著正相关（r=0.002，p
Background and Objectives: This study aimed to explore the changes of gut bacteria in bladder cancer patients. Methods and Study Design: Newly diagnosed bladder cancer patients were recruited. All participants completed a questionnaire about personal behavior and diet. Pyrosequencing of the total genomic DNA extracted from human feces was carried out by Illumina HiSeq 2000. The copy number of target DNA for bacteria was determined by real-time quantitative PCR assay. Fecal short chain fatty acids contents were measured by gas chromatography (GC) analysis. The concentrations of lipopolysaccharide and D-lactic acid in serum were determined by enzyme-linked immunosorbent assay kits. Results: Fruit intake was significantly lower than in healthy controls. The numbers of Clostridium cluster XI and Prevotella in bladder cancer patients decreased. The numbers of domain bacteria and Prevotella were significantly and positively associated with fruit intake (r=0.002, p