Direct Microtubule-Binding by Myosin-10 Orients Centrosomes toward Retraction Fibers and Subcortical Actin Clouds.
Direct Microtubule-Binding by Myosin-10 Orients Centrosomes toward Retraction Fibers and Subcortical Actin Clouds.
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DOI：
10.1016/j.devcel.2015.06.013
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发表时间：
2015-08-10
影响因子：
11.8
通讯作者：
Pellman D
中科院分区：
文献类型：
--
作者：
Kwon M;Bagonis M;Danuser G;Pellman D
中心体的定位对于细胞分裂和发育至关重要。在后生动物细胞中，纺锤体的定位是由皮层下肌动蛋白的动态池控制的，该肌动蛋白根据回缩纤维的位置进行组织。这些肌动蛋白“云”被认为可以对中心体产生拉力并调节纺锤体方向。然而，将星体微管拉向这些肌动蛋白结构的马达尚不清楚。在这里，我们报道了非常规肌球蛋白 Myo10 将来自回缩纤维和皮层下肌动蛋白云的肌动蛋白依赖性力耦合到中心体。 Myo10 介导的中心体定位需要其直接微管结合。对大型微管群的计算图像分析揭示了 Myo10 对微管动力学和微管-皮质相互作用的直接影响。 Myo10 在中心体定位中的作用与动力蛋白不同，但又有重叠。因此，Myo10 在整合肌动蛋白和微管细胞骨架以定位中心体和有丝分裂纺锤体方面发挥着关键作用。
Positioning of centrosomes is vital for cell division and development. In metazoan cells, spindle positioning is controlled by a dynamic pool of subcortical actin that organizes in response to the position of retraction fibers. These actin “clouds” are proposed to generate pulling forces on centrosomes and mediate spindle orientation. However, the motors that pull astral microtubules toward these actin structures are not known. Here, we report that the unconventional myosin, Myo10, couples actin-dependent forces from retraction fibers and subcortical actin clouds to centrosomes. Myo10-mediated centrosome positioning requires its direct microtubule binding. Computational image analysis of large microtubule populations reveals a direct effect of Myo10 on microtubule dynamics and microtubule-cortex interactions. Myo10’s role in centrosome positioning is distinct from, but overlaps with, that of dynein. Thus, Myo10 plays a key role in integrating the actin and microtubule cytoskeletons to position centrosomes and mitotic spindles.