Human papillomavirus infection in oral squamous cell carcinomas from Chilean patients
Human papillomavirus infection in oral squamous cell carcinomas from Chilean patients
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DOI：
10.1016/j.yexmp.2015.06.001
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发表时间：
2015-08-01
影响因子：
3.6
通讯作者：
Aguayo, Francisco
中科院分区：
文献类型：
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作者：
Reyes, Montserrat;Rojas-Alcayaga, Gonzalo;Aguayo, Francisco
人乳头瘤病毒（HPV）是宫颈癌、肛门生殖器癌和口咽癌的一个亚型的致病因子。此外，HPV在口腔癌发生中的作用已被提出，尽管研究结果尚不确定。在这项研究中，使用传统的聚合酶链反应（PCR）和基因分型的特异性PCR和DNA测序，我们分析了80个口腔鳞状细胞癌（OSCC）从智利的主题中的HPV的存在。免疫组化法检测p16、p53、pRb和Ki-67的表达。采用甲基化特异性PCR（MSP）检测CDKN 2A（p16）基因启动子甲基化。HPV序列在9/80（11%）OSCC中发现。发现与p53、pRb、Ki-67和p16水平无统计学显著相关性（分别为p = 0.77; 0.29; 0.83; 0.21）。HPV-16和18型在89例（89%）OSCC中最常见。此外，39%的OSCC中CDKNI 2A（p16）甲基化。这些结果表明，HPV阳性的OSCC是不类似于智利人群中HPV相关肿瘤的分子改变的实体。需要更多的研究来确定HPV在OSCC中的作用。(C)2015 Elsevier Inc. All rights reserved.
Human papillomavirus (HPV) is the causal agent of cervical, anogenital and a subset of oropharyngeal carcinomas. In addition, the role of HPV in oral carcinogenesis has been suggested, although the findings are inconclusive. In this study, using conventional polymerase chain reaction (PCR) and genotyping by specific PCR and DNA sequencing, we analyzed the HPV presence in 80 oral squamous cell carcinomas (OSCCs) from Chilean subjects. In addition, we determined the expression of p16, p53, pRb and Ki-67 using immunohistochemistry (IHC). The CDKN2A (p16) promoter methylation was evaluated using methylation-specific PCR (MSP). HPV sequences were found in 9/80 (11%) OSCCs. Non-statistically significant association with p53, pRb, Ki-67 and p16 levels were found (p = 0.77; 0.29; 0.83; 0.21, respectively). HPV-16 and 18 were the most prevalent HPV genotypes in 8/9 (89%) OSCCs. In addition, CDKNI2A (p16) was methylated in 39% of OSCCs. No association with HPV presence (p = 0.917) was found. These results suggest that HPV positive OSCCs are entities that do not resemble the molecular alterations of HPV-associated tumors in a Chilean population. More studies are warranted to determine the role of HPV in OSCCs. (C) 2015 Elsevier Inc. All rights reserved.