Increase in bacterial community diversity in subsurface aquifers receiving livestock wastewater input
Increase in bacterial community diversity in subsurface aquifers receiving livestock wastewater input
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DOI：
10.1128/aem.66.3.956-965.2000
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发表时间：
2000-03-01
影响因子：
4.4
通讯作者：
Kim, SJ
中科院分区：
文献类型：
--
作者：
Cho, JC;Kim, SJ
尽管对微生物群落多样性进行了深入研究，但哪些微生物种群与群落多样性变化相关的问题尚未通过分子方法得到完全解决。在本研究中，为了调查牲畜废水对地下水细菌群落变化的影响，通过表征其 16S rDNA 序列对地下含水层中的细菌群落进行了分析。克隆的16S核糖体DNA的限制性片段长度多态性（RFLP）模式的相似系数表明，牲畜废水样品中的细菌群落与受污染的含水层样品中的细菌群落关系更密切。此外，群落多样性的计算清楚地表明，牲畜废水和受污染含水层中的细菌群落比未受污染含水层中的细菌群落更加多样化，因此，受污染含水层中细菌群落多样性的增加被认为是由于含有高浓度不同微生物菌群的牲畜废水渗入含水层所致。源自牲畜废水的低 G + C 革兰氏阳性菌群是造成地下水细菌群落变化的原因。不仅在牲畜废水样品中而且在受污染的地下水样品中都出现了与瘤胃相关的序列，这证明了这一点。因此，瘤胃相关序列可以用作地下水（特别是牲畜的）粪便污染的指示序列。
Despite intensive studies of microbial-community diversity, the questions of which kinds of microbial populations are associated with changes in community diversity have not yet been fully solved by molecular approaches. In this study, to investigate the impact of livestock wastewater on changes in the bacterial communities in groundwater, bacterial communities in subsurface aquifers were analyzed by characterizing their 16S rDNA sequences. The similarity coefficients of restriction fragment length polymorphism (RFLP) patterns of the cloned 16S ribosomal DNAs showed that the bacterial communities in livestock wastewater samples were more closely related to those in contaminated aquifer samples. In addition, calculations of community diversity clearly showed that bacterial communities in the livestock wastewater and the contaminated aquifer were much more diverse than those in the uncontaminated aquifer, Thus, the increase in bacterial-community diversity in the contaminated aquifer was assumed to be due to the infiltration of livestock wastewater, containing high concentrations of diverse microbial flora, into the aquifer, Phylogenetic analysis of the sequences from a subset of the RFLP patterns showed that the Cytophaga-Flexibacter-Bacteroides and low-G + C gram-positive groups originating from livestock wastewater were responsible for the change in the bacterial community in groundwater. This was evidenced by the occurrence of rumen-related sequences not only in the livestock wastewater samples but also in the contaminated-groundwater samples. Rumen-related sequences, therefore, can be used as indicator sequences for fecal contamination of groundwater, particularly from livestock.