Isolation and characterization of the human gene encoding Ito:: further diversity by alternative mRNA splicing
Isolation and characterization of the human gene encoding Ito:: further diversity by alternative mRNA splicing
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DOI：
10.1152/ajpheart.1998.275.6.h1963
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发表时间：
1998-12-01
影响因子：
4.8
通讯作者：
Tomaselli, GF
中科院分区：
文献类型：
--
作者：
Kong, W;Po, S;Tomaselli, GF
心脏中的瞬时外向K+电流（I-to）负责复极化的初始阶段和设置心室动作电位的平台电压。最近，Kv4.3已经成为大型哺乳动物（如狗和人类）中I-to的主要候选α亚基基因。我们已经克隆了人Kv4.3同源物，并描述了一种羧基末端剪接变体，其在最后一个跨膜片段之后将具有共有蛋白激酶C（PKC）磷酸化位点的19个氨基酸插入蛋白质中。Kv4.3的编码区由至少5个外显子组成，位于染色体1p13.3上。在基础状态下，剪接变体浴的基本生物物理性质是相同的。
The transient outward K+ current (I-to) in the heart is responsible for the initial phase of repolarization and for setting the plateau voltage of the ventricular action potential. Recently, Kv4.3 has emerged as the leading candidate alpha-subunit gene that underlies I-to in larger mammals such as dogs and humans. we have cloned the human Kv4.3 homolog and describe a carboxyl-terminal splice variant that inserts 19 amino acids with a consensus protein kinase C (PKC) phosphorylation site into the protein after the last membrane-spanning segment. The coding region of Kv4.3 is comprised of at least five exons and is located on chromosome 1p13.3. In the basal state the basic biophysical properties of bath of the splice variants are identical.