Comparison of enterotoxigenic Escherichia coli isolated from surface water and diarrhoeal stool samples in Bangladesh
Comparison of enterotoxigenic Escherichia coli isolated from surface water and diarrhoeal stool samples in Bangladesh
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DOI：
10.1139/w06-098
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发表时间：
2007-01-01
影响因子：
2.8
通讯作者：
Qadri, F.
中科院分区：
文献类型：
--
作者：
Begum, Y. A.;Talukder, K. A.;Qadri, F.
产肠毒素大肠杆菌（ETEC）是导致婴幼儿急性水样腹泻的常见细菌感染原因。尽管孟加拉国的ETEC流行率很高，感染可以通过食物和受污染的水传播，但关于地表水中ETEC的信息有限。我们开展了研究，以从孟加拉国达卡和孟加拉国Matlab的池塘、河流和湖泊的地表水样本中分离ETEC，这些样本来自已知腹泻高发区附近的一个地点。将从水源中分离的ETEC菌株与从这些地区的两家医院的腹泻患者中分离的ETEC菌株进行比较。从30%（45/150）的地表水源样本和19%（518/2700）的临床样本中分离出ETEC。比较了从患者中分离的100株与环境菌株具有相似表型的ETEC菌株的表型和基因型特性。在两种类型的菌株中，ETEC上最常见的O血清群为O 6、O25、O 78、O 115和O 126。ETEC菌株的脉冲场凝胶电泳分析表明，在每种定植因子类型中存在多个ETEC克隆，并且在环境中检测到的一些克隆也从患者粪便中分离出来。菌株显示出多种和相似的抗生素耐药模式。这项研究表明，ETEC在孟加拉国的地表水源中普遍存在，这可能是该病原体在孟加拉国流行的原因。
Enterotoxigenic Escherichia coli (ETEC) is a common cause of bacterial infection leading to acute watery diarrhea in infants and young children. Although the prevalence of ETEC is high in Bangladesh and infections can be spread through food and contaminated water, limited information is available about ETEC in the surface water. We carried out studies to isolate ETEC from surface water samples from ponds, rivers, and a lake from a site close to field areas known to have a high incidence of diarrhea in Dhaka, Bangladesh, and Matlab, Bangladesh. ETEC strains isolated from the water sources were compared with ETEC strains isolated from patients with diarrhea at two hospitals in these areas. ETEC were isolated from 30% (45 of 150) of the samples from the surface water sources and 19% (518 of 2700) of the clinical specimens. One hundred ETEC strains isolated from patients with similar phenotypes as the environmental strains were compared for phenotypic and genotypic properties. The most common O serogroups on ETEC were O6, O25, O78, O115, and O126 in both types of strains. Pulsed-field gel electrophoresis analyses of the ETEC strains showed that multiple clones of ETEC were present within each colonization factor type and that some clones detected in the environment were also isolated from the stools of patients. The strains showed multiple and similar antibiotic resistance patterns. This study shows that ETEC is prevalent in surface water sources in Bangladesh suggesting a possible reason for the endemicity of this pathogen in Bangladesh.