Biofilm formation and genotyping of Candida haemulonii, Candida pseudohaemulonii, and a proposed new species (Candida auris) isolates from Korea
Biofilm formation and genotyping of Candida haemulonii, Candida pseudohaemulonii, and a proposed new species (Candida auris) isolates from Korea
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DOI：
10.3109/13693786.2010.493563
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发表时间：
2011-01-01
期刊：
影响因子：
2.9
通讯作者：
Ryang, Dong Wook
中科院分区：
文献类型：
--
作者：
Oh, Bong Joon;Shin, Jong Hee;Ryang, Dong Wook
最近在五家韩国医院发现了海默洛尼假丝酵母菌及其近缘种。我们检测了这些菌株的生物被膜形成，并用脉冲场凝胶电泳法(PFGE)评估了它们的基因关联性。这项研究首次表明，所有假贺穆伦假丝酵母菌的血液分离株都能在含葡萄糖的介质中形成显著的生物膜。对未消化的基因组DNA进行的PFGE分析表明，从两家医院的7名患者中分离到的伪海毛念珠菌有5种相同的带型，而从3家医院的患者那里获得的一个新物种(Candida Auris)的15株菌株有7种相同的带型，这表明其中一些分离株可能与克隆传播有关。
Emergence of Candida haemulonii and closely related species at five Korean hospitals has been recently described. We examined biofilm formation by these isolates and assessed their genotypic relatedness by pulsed-field gel electrophoresis (PFGE). This study is the first to show that all bloodstream isolates of Candida pseudohaemulonii can form significant biofilms in glucose-containing medium. PFGE of NotI-digested genomic DNA revealed that C. pseudohaemulonii isolates recovered from seven patients in two hospitals shared five patterns, and that 15 isolates of a proposed new species (Candida auris) obtained from patients at three hospitals shared seven patterns, suggesting that some of these isolates may be related to clonal transmission.