Estimation of Seroprevalence of the Pandemic H1N1 2009 Influenza Virus Using a Novel Virus-Free ELISA Assay for the Detection of Specific Antibodies
Estimation of Seroprevalence of the Pandemic H1N1 2009 Influenza Virus Using a Novel Virus-Free ELISA Assay for the Detection of Specific Antibodies
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DOI：
10.1089/vim.2010.0137
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发表时间：
2011-06-01
期刊：
影响因子：
2.2
通讯作者：
Tsakris, Athanassios
中科院分区：
文献类型：
--
作者：
Mavrouli, Maria D.;Routsias, John G.;Tsakris, Athanassios
2009年H1N1甲型流感于2009年4月出现，并迅速蔓延到世界各地。在希腊，2009年5月18日报告了第一例H1N1大流行病例，而在2009年7月初注意到病例数量大幅增加。由于需要监测希腊人口的免疫状况，我们开发了一种无病毒ELISA，可以特异性识别人血清中的2009年H1N1大流行流感病毒抗体。该方法基于使用分别源自2009年大流行毒株的血凝素和神经氨酸酶的合成肽（H1-pep和N1-pep），并区分猪源甲型流感/加州/14/2009（H1 N1）和季节性甲型流感病毒。在2009年5月、11月和12月期间，从271名健康献血者中获得血清样本。在5月、11月和12月期间收集的血清中，分别在7.4%、13.8%和19.3%的供体中检测到针对肽H1-pep的IgG抗体，而分别在5.3%、9.6%和16.9%的供体中检测到针对肽N1-pep的IgG抗体。免疫测定的应用表明，在希腊H1N1大流行期间，抗H1-pep和抗N1-pep IgG抗体的流行率呈时间依赖性增加。该方法还可以指示免疫人与易受大流行性H1N1毒株感染的人的区分，以及建立有效的疫苗接种计划。
The pandemic H1N1 2009 influenza A emerged in April 2009 and spread rapidly all over the world. In Greece, the first case of the pandemic H1N1 was reported on May 18, 2009, while a considerable increase in the number of cases was noticed at the beginning of July 2009. The need for surveillance of the immune status of the Greek population led us to develop a virus-free ELISA that specifically recognizes pandemic H1N1 2009 influenza virus antibodies in human sera. The method is based on the use of synthetic peptides (H1-pep and N1-pep) that are derived from the hemagglutinin and neuraminidase of the 2009 pandemic strain, respectively, and differentiate the swine-origin influenza A/California/14/2009 (H1N1) from the seasonal influenza A viruses. Serum samples were obtained from 271 healthy blood donors during May, November, and December 2009. Among sera collected during May, November, and December, IgG antibodies against the peptide H1-pep were detected in 7.4, 13.8, and 19.3% of the donors, respectively, while IgG antibodies against the peptide N1-pep were detected in 5.3, 9.6, and 16.9% of the donors, respectively. The application of the immunoassay indicated a time-dependent increase of the prevalence of anti-H1-pep and anti-N1-pep IgG antibodies during the pandemic H1N1 outbreak in Greece. The method could be also indicative for the discrimination of immune persons from those susceptible to infection with the pandemic H1N1 strain, as well as for the establishment of effective vaccination programs.