DNA polymerase-mediated DNA synthesis on a TNA template
DNA polymerase-mediated DNA synthesis on a TNA template
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DOI：
10.1021/ja028589k
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发表时间：
2003-01-29
影响因子：
15
通讯作者：
Szostak, JW
中科院分区：
文献类型：
--
作者：
Chaput, JC;Ichida, JK;Szostak, JW
TNA或苏糖核酸能够与DNA、RNA和TNA进行沃森-克里克碱基配对;再加上其化学结构简单，这表明TNA可能是RNA的祖先。作为开发必要的分子工具，以调查TNA的功能能力，在体外选择的第一步，我们已经筛选了各种DNA聚合酶的活性TNA模板。我们报告说，尽管有一个重复单元，是一个原子比DNA短，几个聚合酶表现出令人惊讶的良好能力，复制有限的TNA延伸。
TNA, or threose nucleic acid, is capable of Watson−Crick base pairing with DNA, RNA, and TNA; coupled with its chemical simplicity, this suggests that TNA is a possible progenitor of RNA. As an initial step toward developing the molecular tools necessary to investigate the functional capabilities of TNA by in vitro selection, we have screened a variety of DNA polymerases for activity on a TNA template. We report that despite having a repeating unit that is one atom shorter than that of DNA, several polymerases showed surprisingly good ability to copy limited stretches of TNA.