Bulged adenosine influence on the RNA duplex conformation in solution
Bulged adenosine influence on the RNA duplex conformation in solution
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DOI：
10.1021/bi7024904
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发表时间：
2008-05-06
期刊：
影响因子：
2.9
通讯作者：
Gdaniec, Zofia
中科院分区：
文献类型：
--
作者：
Popenda, Lukasz;Adamiak, Ryszard W.;Gdaniec, Zofia
RNA单凸起基序是在若干互补碱基对链内的未配对残基。为了深入了解腺苷凸起在RNA双工上引起的结构变化，通过核磁共振光谱测定了含有单个腺嘌呤凸起的RNA双工（5'-GCAGAAGAGCG-3'/5'-CGCUCUCUGC-3‘）和含有所有沃森-克里克碱基对的参考双工（5’ gcagagagcg -3'/5'-CGCUCUCUGC-3'）的溶液结构。参考双相结构是一个规则的右手螺旋，具有a型螺旋的所有属性。在凸起的双体中，单个腺嘌呤凸起堆积成螺旋状，凸起区域形成明确的结构。利用计算得到的螺旋参数对两种结构进行了分析。由未配对残基进入螺旋结构所引起的畸变会传播到整个结构中，并表现为碱基对倾角和x位移的减少。凸起的腺嘌呤A5通过与y邻近残基G4的有效堆积而稳定螺旋内位置。
The RNA single bulge motif is an unpaired residue within a strand of several complementary base pairs. To gain insight into structural changes induced by the presence of the adenosine bulge on RNA duplex, the solution structures of RNA duplex containing a single adenine bulge (5'-GCAGAAGAGCG-3'/5'-CGCUCUCUGC-3') and a reference duplex with all Watson-Crick base pairs (5'GCAGAGAGCG-3'/5'-CGCUCUCUGC-3') have been determined by NMR spectroscopy. The reference duplex structure is a regular right-handed helix with all of the attributes of an A-type helix. In the bulged duplex, single adenine bulge stacks into the helix, and the bulge region forms a well-defined structure. Both structure's were analyzed by the use of calculated helical parameters. Distortions induced by the accommodation of unpaired residue into the helical structure propagate over the entire structure and,are manifested as the reduced base pairs inclination and x-displacement. Intrahelical position of bulged adenine A5 is stabilized by efficient stacking with Y-neighboring residues G4.