Fluorescence-tunable probe for antioxidant capacity assay based on dopamine self-polymerization on graphitic carbon nitride
Fluorescence-tunable probe for antioxidant capacity assay based on dopamine self-polymerization on graphitic carbon nitride
复制标题
基于石墨碳氮化物多巴胺自聚合的荧光可调探针抗氧化能力测定
DOI：
10.1016/j.snb.2018.02.062
复制
发表时间：
2018-06-01
影响因子：
8.4
通讯作者：
Yao, Jian-Nian
中科院分区：
文献类型：
--
作者：
Liu, Jing-Jing;Chen, Zhi-Tao;Yao, Jian-Nian
氧化应激涉及多种炎症性和退行性疾病，已成为大多数生物医学学科和许多类型的临床研究的热点。抗氧化剂可以保护生物系统免受氧化应激的有害影响。通过检测机体抗氧化能力，从另一个侧面反映机体对氧化应激的监测作用。因此，测定生物体液的抗氧化能力就显得尤为重要。本文首次基于聚多巴胺猝灭荧光石墨氮化碳（g-C3N4）开发了一种荧光可调探针，用于评估生物体液的抗氧化能力。多巴胺（DA）可在g-C3N4表面自聚合形成聚多巴胺（PDA）均匀包覆层，在聚多巴胺（PDA）包覆层中，g-C3N4的荧光被PDA层有效猝灭，呈现荧光“关闭”状态。然而，在抗氧化剂的存在下，DA的自聚合作用可被有效抑制，导致荧光“开启”状态。因此，检测抗氧化剂可以通过监测g-C3N4的荧光发射来实现。此外，该传感系统具有较高的灵敏度和选择性，成功地应用于生物流体中抗氧化能力的评估，结果与常用的抗氧化检测方法吻合较好。(C) 2018 Elsevier B.V.版权所有
Oxidative stress, which involved in several inflammatory and degenerative diseases, has become interesting in most of biomedical disciplines and many types of clinical research. Antioxidants can protect biological systems from the harmful effects of oxidative stress. It can reflect the monitor effect to oxidative stress from another aspect by detecting the antioxidant capacity. Therefore, it is particularly significant to assay the antioxidant capacity of biological fluids. Herein, for the first time a fluorescence-tunable probe was developed based on polydopamine-quenched fluorescent graphitic carbon nitride (g-C3N4) for assessing the antioxidant capacity of biological fluids. Dopamine (DA) can self-polymerize on the surface of g-C3N4 to produce a polydopamine (PDA) uniform coating layer in which the fluorescence of g-C3N4 is efficiently quenched by the PDA layer, showing the fluorescence "off" state. However, in the presence of antioxidants, the self -polymerized effect of DA can be effectively inhibited and leading to the fluorescence "on" state. Therefore, detecting antioxidants can be achieved by monitoring the fluorescent emission of g-C3N4. Moreover, endowed with high sensitivity and selectivity, the proposed sensing system was successfully applied to assessment antioxidant capacity in biological fluid and the results matched well with commonly used antioxidant detection method. (C) 2018 Elsevier B.V. All rights reserved.