Pathogen‐induced vascular gels: Ethylene as a host intermediate
Pathogen‐induced vascular gels: Ethylene as a host intermediate
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病原体诱导的血管凝胶：乙烯作为宿主中间体
DOI：
10.1111/j.1399-3054.1983.tb06282.x
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
J. Devay
中科院分区：
文献类型：
--
作者：
G. Vandermolen;J. Labavitch;L. Strand;J. Devay
一种尖孢镰刀菌培养6天的无细胞培养液。Cubense被加工成：(1)多相混合酶，(2)纯化的聚半乳糖醛酸酶(PG)，(3)部分纯化的聚半乳糖醛酸裂解酶和(4)β-1，4-木聚糖酶。当这些制剂被引入到蓖麻叶中时，每一种制剂都能够促进阻碍血管系统的凝胶的形成。将蓖麻叶暴露在乙烯(3ppm)中也会引发凝胶形成。外植叶在酶混合物和PG的作用下产生乙烯。当阻止酶反应产生乙烯时，血管凝胶的形成不会发生。
A cell free culture filtrate from 6-day cultures of Fusarium oxysporum f. sp. cubense was processed to give: (1) a heterogeneous enzyme mixture, (2) purified polygalacturonase (PG), (3) partially-purified polygalacturonate lyase and (4) β-1,4-xylanase. When introduced into explanted castor bean leaves each of these preparations was able to promote the formation of vascular system-obstructing gels. Exposure of castor bean leaves to ethylene (3 ppm) also triggered gel formation. Explanted leaves produced ethylene in response to the enzyme mixture and PG. Vascular gel formation did not occur when ethylene production in response to enzymes was prevented.