Secretion of the virulence-associated Campylobacter invasion antigens from Campylobacter jejuni requires a stimulatory signal
Secretion of the virulence-associated Campylobacter invasion antigens from Campylobacter jejuni requires a stimulatory signal
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DOI：
10.1086/320704
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发表时间：
2001-06-01
影响因子：
6.4
通讯作者：
Konkel, ME
中科院分区：
文献类型：
--
作者：
Rivera-Amill, V;Kim, BJ;Konkel, ME
空肠弯曲菌是人类肠道疾病的常见病原。已有的研究表明，C.空肠在与上皮细胞共培养时合成一组新的蛋白质，其中一些是分泌的。分泌的蛋白质被统称为弯曲杆菌侵袭抗原（Cia蛋白）。代谢标记实验表明，Cia蛋白的合成和分泌是可分离的，分泌是这些过程的限速步骤。另外的工作表明，Cia蛋白的合成是响应于胆盐和各种真核宿主细胞组分而诱导的。宿主细胞组分也可诱导Cia蛋白分泌。培养C.空肠在添加有脱氧胆酸盐的平板上延迟氯霉素对C.空肠侵入，如通过庆大霉素保护测定所判断的。这些数据表明，Cia蛋白编码基因的协调表达受到环境调节。
Campylobacter jejuni are a common cause of human diarrheal illness. Previous work has demonstrated that C. jejuni synthesize a novel set of proteins upon coculturing with epithelial cells, some of which are secreted. The secreted proteins have been collectively referred to as Campylobacter invasion antigens (Cia proteins). Metabolic labeling experiments revealed that Cia protein synthesis and secretion are separable and that secretion is the rate-limiting step of these processes. Additional work indicated that Cia protein synthesis is induced in response to bile salts and various eukaryotic host cell components. Host cell components also can induce Cia protein secretion. Culturing C. jejuni on plates supplemented with the bile salt deoxycholate retarded the inhibitory effect of chloramphenicol on C. jejuni invasion, as judged by the gentamicin-protection assay. These data suggest that the coordinate expression of the genes encoding the Cia proteins is subject to environmental regulation.