Regulation of the calcium-sensing receptor expression by 1,25-dihydroxyvitamin D3, interleukin-6, and tumor necrosis factor alpha in colon cancer cells.
Regulation of the calcium-sensing receptor expression by 1,25-dihydroxyvitamin D3, interleukin-6, and tumor necrosis factor alpha in colon cancer cells.
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DOI：
10.1016/j.jsbmb.2013.10.015
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发表时间：
2014-10
影响因子：
4.1
通讯作者：
Kallay, Eniko
中科院分区：
文献类型：
--
作者：
Fetahua, Irfete S.;Hummel, Doris M.;Manhardt, Teresa;Aggarwal, Abhishek;Baumgartner-Parzer, Sabina;Kallay, Eniko
关键词：
1,25 Dihydroxyvitamin D3 induces the expression of CaSR in Caco2/AQ and Coga1A cells. TNFα is the main driver of CaSR expression in Coga1A. In Caco2/AQ cells 1,25 dihydroxyvitamin D3 counteracts the action of TNFα and IL-6. Anti-proliferative effects of calcium in the colon are mediated, at least in part, via the calcium-sensing receptor (CaSR), a vitamin D target gene. The expression of CaSR decreases during colorectal tumor progression and the mechanisms regulating its expression are poorly understood. The CaSR promoter harbors vitamin D elements responsive to 1,25-dihydroxyvitamin D3 (1,25D3) and NF-κB, STAT, and SP1 binding sites accounting for responsiveness to proinflammatory cytokines. Therefore, in the current study we investigated the impact of 1,25D3, tumor necrosis factor alpha (TNFα), and interleukin (IL)-6 on CaSR expression in a differentiated (Caco2/AQ) and in a moderately differentiated (Coga1A) colon cancer cell line. 1,25D3 induced CaSR expression in both cell lines. Treatment with TNFα was accompanied by a 134-fold induction of CaSR in Coga1A (p < 0.01). In Caco2/AQ cells the expression of CaSR was upregulated also by IL-6 (3.5-fold). Our data demonstrated transcriptional and translational activation of the CaSR by 1,25D3, TNFα, and IL-6 in a time- and cell line-dependent manner. This article is part of a Special Issue entitled ‘16th Vitamin D Workshop’.
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影响因子：
6.4
作者：
Hoebaus, Julia;Hummel, Doris M.;Thiem, Ursula;Fetahu, Irfete S.;Aggarwal, Abhishek;Muellauer, Leonhard;Heller, Gerwin;Egger, Gerda;Mesteri, Ildiko;Baumgartner-Parzer, Sabina;Kallay, Enikoe
通讯作者：
Kallay, Enikoe
影响因子：
3.8
作者：
Brozek, Wolfgang;Bises, Giovanna;Peterlik, Meinrad
通讯作者：
Peterlik, Meinrad
DOI：
10.1016/j.bpg.2011.10.001
发表时间：
2011-08-01
影响因子：
3.2
作者：
Zhang, Xuehong;Giovannucci, Edward
通讯作者：
Giovannucci, Edward
影响因子：
4.1
作者：
BEAULIEU, JF;QUARONI, A
通讯作者：
QUARONI, A
影响因子：
4.8
作者：
Chikatsu, N;Fukumoto, S;Fujita, T
通讯作者：
Fujita, T