A-TYPE POTASSIUM CHANNELS EXPRESSED FROM SHAKER LOCUS CDNA
A-TYPE POTASSIUM CHANNELS EXPRESSED FROM SHAKER LOCUS CDNA
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DOI：
10.1073/pnas.85.15.5723
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发表时间：
1988-08-01
影响因子：
11.1
通讯作者：
RUDY, B
中科院分区：
文献类型：
--
作者：
IVERSON, LE;TANOUYE, MA;RUDY, B
A 型 K+ 电流在非洲爪蟾卵母细胞中表达，该卵母细胞注射了体外合成的果蝇 Shaker (Sh) 基因座 cDNA 转录本。单个 Sh 基因产物（可能为多聚体）足以形成功能性 A 通道。各种 Sh RNA 表达具有不同动力学特性的 A 电流。结构-功能关系的分析表明，Sh 多肽的保守中心区域决定离子选择性和整体通道行为，而不同的氨基和羧基末端可以改变通道动力学。 Sh 基因转录本的选择性剪接可能为 K+ 通道多样性的产生提供一种机制。
A-type K+ currents are expressed in Xenopus oocytes injected with in vitro-synthesized transcripts from cDNAs for the Drosophila Shaker (Sh) locus. A single Sh gene product, possibly as a multimer, is sufficient for formation of functional A channels. Various Sh RNAs express A currents with distinct kinetic properties. An analysis of structure-function relationships shows that the conserved central region of Sh polypeptides determines ionic selectivity and overall channel behavior, whereas the divergent amino and carboxyl termini can modify channel kinetics. Alternative splicing of Sh gene transcripts may provide one mechanism for the generation of K+ channel diversity.