Stroke Alters Respiratory Burst in Neutrophils and Monocytes
Stroke Alters Respiratory Burst in Neutrophils and Monocytes
复制标题
DOI：
10.1161/strokeaha.113.003342
复制
发表时间：
2014-03-01
期刊：
影响因子：
8.3
通讯作者：
Vogelgesang, Antje
中科院分区：
文献类型：
--
作者：
Ruhnau, Johanna;Schulze, Karsten;Vogelgesang, Antje
背景和目的脑卒中诱导的免疫改变使患者易患感染。虽然中风和适应性免疫系统之间的关系已被详细研究，但到目前为止，还不清楚形成第一道抗菌防御的先天免疫系统是否也在中风患者中受损。因此，我们调查是否趋化性，吞噬作用，氧化爆发，防御素脱颗粒，和NETosis在单核细胞和中性粒细胞中的改变，中风患者与controls.Methods招募了63例急性缺血性中风患者在12小时内的症状发作，血液采样入院，并在第1，3，5和7天。还招募了37名年龄匹配的对照。在体外测定细胞迁移、吞噬作用和吞噬细胞的氧化爆发。人中性粒细胞肽1至3和血清metaneosorbent水平测定酶联免疫吸附试验，和NETosis定量免疫组化。结果细菌杀伤，氧化爆发，和NETosis所需的关键机制，从中风患者的样品中显着减少与对照组相比，而迁移，吞噬功能，和防御素的生产仍然是未受损的单核细胞和粒细胞从patients.Conclusions中风引起的免疫改变，包括损害的第一线防御专门的吞噬细胞对细菌。这些变化增强了对获得性感染的易感性的假设得到了我们的观察结果的支持，即在入院时单核细胞中的氧化爆发在中风患者中受到更多的损害，随后发生中风相关感染。
Background and Purpose Stroke-induced immune alterations predispose patients to infections. Although the relationship between stroke and the adaptive immune system has been investigated in detail, to date it is unknown whether the innate immune system, which forms the first line of antibacterial defense, is also impaired in patients with stroke. Therefore, we investigated whether chemotaxis, phagocytosis, oxidative burst, degranulation of defensins, and NETosis in monocytes and in neutrophil granulocytes are altered in patients with stroke compared with controls.Methods Sixty-three patients having acute ischemic stroke were recruited within 12 hours of symptom onset; blood was sampled on admission and on days 1, 3, 5, and 7. Thirty-seven age-matched controls were also recruited. Cell migration, phagocytosis, and oxidative burst of phagocytes were determined in vitro. Human neutrophil peptides 1 to 3 and serum metanephrine levels were measured by enzyme-linked immunosorbent assay, and NETosis was quantified by immunohistochemistry.Results The key mechanisms required for bacterial killing, oxidative burst, and NETosis were significantly reduced in samples taken from patients with stroke compared with controls, whereas migration, phagocytic function, and defensin production remained unimpaired in monocytes and granulocytes from patients with stroke.Conclusions Stroke-induced immune alterations include impairment of the first-line defense performed by specialized phagocytes against bacteria. The hypothesis that these changes enhance susceptibility to acquired infections is supported by our observation that on admission oxidative burst in monocytes was more impaired in patients with stroke with subsequent stroke-associated infections.