Influence of riboflavin on the oxidation kinetics of unsaturated fatty acids at the air/aqueous interface revealed by sum frequency generation vibrational spectroscopy
Influence of riboflavin on the oxidation kinetics of unsaturated fatty acids at the air/aqueous interface revealed by sum frequency generation vibrational spectroscopy
复制标题
和频振动光谱揭示核黄素对空气/水界面不饱和脂肪酸氧化动力学的影响
DOI：
10.1039/c8cp00975a
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发表时间：
2018
影响因子：
3.3
通讯作者：
Lu Zhou
中科院分区：
文献类型：
--
作者：
Ma Yingxue;Hou Jian;Hao Wenying;Liu Jianchuan;Meng Lingwei;Lu Zhou
核黄素是一种常见的营养素，也被称为维生素B2，已知在预防脂质过氧化方面可能发挥重要作用。然而，详细的抗氧化机制，特别是核黄素对生物界面脂质氧化的影响，尚未得到充分探讨。在目前的研究中，核黄素分子对单不饱和顺式-11-二十碳烯酸（EA）在空气/水界面的氧化动力学的影响进行了系统的研究，使用和频产生振动光谱（SFG-VS）。结果发现，在核黄素的存在下，在含水亚相中的界面EA分子的氧化速率可以减少约两到三倍。进一步SFG-VS测量下的氮气吹扫气体的保护下，更紧密地包装和有序的单层结构形成的核黄素分子的表面吸附，使CC键不易接近的气相氧化物种。这些结果表明，核黄素在生物膜附近的抗氧化机制可能不一定涉及其他还原剂。它们还显示了界面分子结构在生物相关化学反应中的重要性。
Riboflavin, a common nutrient also known as vitamin B2, is known to potentially play important roles in preventing lipid peroxidations. However, the detailed antioxidant mechanisms, especially the influence of riboflavin on lipid oxidations at biological interfaces, have not yet been fully explored. In the current study, the effect of riboflavin molecules on the oxidation kinetics of monounsaturated cis-11-eicosenoic acid (EA) at the air/water interface was systematically investigated using sum frequency generation vibrational spectroscopy (SFG-VS). It was discovered that the oxidation rates of the interfacial EA molecules can be reduced by about two to three times in the presence of riboflavin in the aqueous subphase. Further SFG-VS measurements under the protection of nitrogen purging gas showed that more tightly packed and ordered monolayer structures were formed by the surface adsorption of riboflavin molecules, making the CC bonds less accessible to the gas phase oxidative species. These results suggested that the antioxidant mechanism for riboflavin in the vicinity of biomembranes may not necessarily involve other reducing agents. They also show the great importance of interfacial molecular structures in biologically relevant chemical reactions.