Fabrication of a colorimetric biosensing platform for the detection of protein-DNA interaction.
Fabrication of a colorimetric biosensing platform for the detection of protein-DNA interaction.
复制标题
构建用于检测蛋白质-DNA 相互作用的比色生物传感平台。
DOI：
10.1016/j.bios.2013.02.028
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发表时间：
2013
影响因子：
12.6
通讯作者：
Nandi Zhou
中科院分区：
文献类型：
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作者：
Zonghuang Ye;Wei Zhang;Lili Liu;Guifang Chen;Zhongming Shen;Nandi Zhou
蛋白质-DNA相互作用在许多细胞过程中起着重要的作用，迫切需要有效的方法来监测这种相互作用。有鉴于此，我们提出了一种简单的无标记比色平台来检测蛋白质与DNA的相互作用。蛋白质-DNA偶联以及类似过氧化物酶的脱氧核酸酶和核酸外切酶被精心地结合到一个集成的生物传感系统中。该策略除了简单、高效外，还具有通用性强的优点，适用于不同蛋白质-DNA对的检测。在我们的实验中，我们方法的有效验证可以得到两个不同的蛋白质-DNA对(雌激素受体α和核因子kappa B)的支持。实验结果表明，DNAzyme能够产生明显的读出信号来监测蛋白质-DNA对。此外，随着探针中DNA结合序列的取代，该方法可以扩展到检测蛋白质-DNA相互作用的通用平台。
Protein–DNA interaction plays important roles in many cellular processes, and there is an urgent demand for valid methods to monitor the interaction. In view of this, we propose a simple label-free colorimetric platform for the detection of protein–DNA interaction. Protein–DNA couples together with peroxidase-mimicking DNAzyme and exonuclease are elaborately incorporated into an integrated biosensing system. Besides the simplicity and efficiency, the strategy also has a great advantage for its universality in the detection of different protein–DNA couples. In our experiments, effective validation of our approach can be supported by two different protein–DNA couples (estrogen receptor α and nuclear factor kappa B). Experimental results show that the DNAzyme is competent to give rise to evident readout signals to monitor protein–DNA couples. Furthermore, with the substitution of DNA binding sequence in the probe, this method could be extended to a general platform for the detection of protein–DNA interaction.
影响因子：
15
作者：
Vallee-Belisle, Alexis;Bonham, Andrew J.;Reich, Norbert O.;Ricci, Francesco;Plaxco, Kevin W.
通讯作者：
Plaxco, Kevin W.
DOI：
10.1074/mcp.m900275-mcp200
发表时间：
2010-03
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
Yin X;Cuello F;Mayr U;Hao Z;Hornshaw M;Ehler E;Avkiran M;Mayr M
通讯作者：
Mayr M