Accumulation of soluble and wall-bound indolic metabolites in Arabidopsis thaliana leaves infected with Virulent or avirulent Pseudomonas syringae pathovar tomato strains
Accumulation of soluble and wall-bound indolic metabolites in Arabidopsis thaliana leaves infected with Virulent or avirulent Pseudomonas syringae pathovar tomato strains
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DOI：
10.1073/pnas.021551098
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发表时间：
2001-01-16
影响因子：
11.1
通讯作者：
Hahlbrock, K
中科院分区：
文献类型：
--
作者：
Hagemeier, J;Schneider, B;Hahlbrock, K
研究了番茄丁香假单胞菌侵染拟南芥叶片后，几种主要可溶性、壁结合、碱水解化合物的化学结构和积累动力学。所有鉴定的积累产物在结构上都与色氨酸相关。可溶性物质以色氨酸、β - d -葡萄糖吡喃基吲哚-3-羧酸、6-羟基吲哚-3-羧酸6- o - β - d -葡萄糖吡喃苷为主。还有吲哚植物抗菌素camalexin。在这些条件下检测到的单一主要累积壁成分是吲哚-3-羧酸。这些化合物的含量均以较快的速度增加，不亲和对照中camalexin和吲哚-3-羧酸的含量明显高于亲和对照。芥交互。可溶性提取物中仅有的三种显著的苯丙衍生物表现不同。两种山奈酚苷基本未受影响，苹果酸sinapoyl在细菌感染后急剧下降，其时间过程与色氨酸相关产物的积累呈负相关。对于受感染的野生型和tt4（没有山奈酚苷）或fah1（没有苹果酸sinapoyl）突变株，可溶性和壁结合化合物的积累模式以及抗病表型基本相同。在受伤或衰老的拟南芥叶片中积累了大量不同的产品组合。迄今为止发现的任何一种感染诱导化合物似乎都不太可能在对丁香假单胞菌的抗性反应中起决定性作用。
The chemical structures and accumulation kinetics of several major soluble as well as wall-bound, alkali-hydrolyzable compounds induced upon infection of Arabidopsis thaliana leaves with Pseudomonas syringae pathovar tomato were established. All identified accumulating products were structurally related to tryptophan. Most prominent among the soluble substances were tryptophan, beta -D-glucopyranosyl indole-3-carboxylic acid, 6-hydroxyindole-3-carboxylic acid 6-O-beta -D-glucopyranoside. and the indolic phytoalexin camalexin. The single major accumulating wall component detectable under these conditions was indole-3-carboxylic acid. All of these compounds increased more rapidly, and camalexin as well as indole-3-carboxylic acid reached much higher levels, in the incompatible than in the compatible P. syringae/A. thaliana interaction. The only three prominent phenylpropanoid derivatives present in the soluble extract behaved differently. Two kaempferol glycosides remained largely unaffected, and sinapoyl malate decreased strongly upon bacterial infection with a time course inversely correlated with that of the accumulating tryptophan-related products. The accumulation patterns of both soluble and wall-bound compounds, as well as the disease resistance phenotypes, were essentially the same for infected wild-type and tt4 (no kaempferol glycosides) or fah1 (no sinapoyl malate) mutant plants. Largely different product combinations accumulated in wounded or senescing A. thaliana leaves. It seems unlikely that any one of the infection-induced compounds identified so far has a decisive role in the resistance response to P. syringae.