Chemotaxis-Instructed Intracellular Staphylococcus aureus Infection Detection by a Targeting and Self-Assembly Signal-Enhanced Photoacoustic Probe
Chemotaxis-Instructed Intracellular Staphylococcus aureus Infection Detection by a Targeting and Self-Assembly Signal-Enhanced Photoacoustic Probe
复制标题
通过靶向和自组装信号增强光声探针进行趋化指导的细胞内金黄色葡萄球菌感染检测。
DOI：
10.1021/acs.nanolett.8b02286
复制
发表时间：
2018-10-01
期刊：
影响因子：
10.8
通讯作者：
Wang, Hao
中科院分区：
文献类型：
--
作者：
Cai, Qian;Fei, Yue;Wang, Hao
金黄色葡萄球菌在吞噬细胞内的侵袭和存活被认为是导致金黄色葡萄球菌感染治疗失败和潜在抗生素耐药性的机制之一。感染吞噬细胞的检测对于指导抗生素治疗和减少耐药性发挥着重要作用。开发一种灵敏且特异的成像探针来可视化细胞内细菌是相当具有挑战性的。在这项工作中，我们报道了一种光声剂（MPC），它能够通过动态过程检测细胞内金黄色葡萄球菌感染，包括（i）主动靶向和内化到巨噬细胞中，（ii）感染巨噬细胞中caspase-1的特异性分子剪裁，以及（iii）由于分子自组装而增强光声（PA）信号。 “刺激诱导组装”剂 (MPC) 的单位面积 PA 信号比主动靶向控制剂 (MPSC) 增加了 2 倍以上。最后，基于这种方法，感染部位的平均 PA 信号比对照部位增强了约 2.6 倍。我们设想这种 PA 造影剂可能为细胞内细菌感染的选择性和灵敏诊断提供一种新方法。
Intracellular invasion and the survival of Staphylococcus aureus in phagocytic cells has been regarded as one of the mechanisms that leads to the treatment failure of S. aureus infection and potential antibiotic resistance. The detection of infected phagocytic cells plays an important role in guiding antibiotic treatment and in reducing drug resistance. The development of a sensitive and specific imaging probe to visualize the intracellular bacteria is quite challenging. In this work, we report a photoacoustic agent (MPC) that is able to detect intracellular S. aureus infection through a dynamic process, including (i) active targeting and internalization into macrophage cells, (ii) specific molecular tailoring by caspase-1 in infected macrophage cells, and (iii) enhancement of the photoacoustic (PA) signal owing to molecular self-assembly. The PA signal per area of the "stimuli-induced assembly" agent (MPC) increases more than 2-fold over that of the active targeting control agent (MPSC). Finally, based on this approach, the average PA signal in the infected site is enhanced by approximately 2.6-fold over that of the control site. We envision that this PA contrast agent may provide a new approach for the selective and sensitive diagnosis of an intracellular bacterial infection.