Arguments against the prostatic origin of the R-3327 Dunning H tumor
Arguments against the prostatic origin of the R-3327 Dunning H tumor
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反对 R-3327 Dunning H 肿瘤前列腺起源的争论
DOI：
10.1007/bf02899660
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
G. Aumüller
中科院分区：
文献类型：
--
作者：
Heinz;U. Rausch;M. Steinhoff;J. Seitz;M. Bacher;M. Papotti;G. Bussolati;P. Tuohimaa;G. Aumüller
Dunning瘤最初被描述为大鼠背侧前列腺癌，长期以来一直被用作前列腺癌的实验模型。我们最近提出了一些形态学的发现，是不相容的邓宁肿瘤的H-亚系的前列腺起源。本文研究了大鼠R-3327 Dunning H肿瘤中前列腺和乳腺的生化和免疫组化标记。将H肿瘤的碎片接种到雄性或哺乳期雌性大鼠中。Dunning肿瘤提取物的蛋白电泳图谱与乳腺的蛋白电泳图谱比背外侧前列腺的蛋白电泳图谱更相似。蛋白质选择性地出现后，代谢标记在哺乳期大鼠生长的邓宁肿瘤对应于标记的蛋白质在乳腺从相同的动物。在Dunning肿瘤中，不能用化学方法检测到侧前列腺（SVS II）和背前列腺（转氨酶）的典型分泌蛋白。肿瘤提取物的Western印迹研究和肿瘤RNA制备物的狭缝印迹分析证实了Dunning肿瘤中不存在SVS II和前列腺特异性转氨酶。另一方面，乳腺蛋白，如乳脂球膜蛋白，乳过氧化物酶和乳白蛋白的存在下检测到邓宁肿瘤免疫组化和蛋白质印迹，但不存在的背外侧前列腺。转铁蛋白mRNA，表达在男性泌尿生殖道，也在肝脏和其他组织中，检测到乳腺和邓宁肿瘤，但不是在前列腺背外侧。Dunning瘤中乳腺分泌性β-酪蛋白的缺乏与肿瘤中Ha-ras癌基因表达升高有关，先前报道抑制酪蛋白表达。研究结果清楚地表明，前列腺不可能是邓宁肿瘤的起源，目前正在用于前列腺癌的研究。因此，该肿瘤的前列腺腺癌命名无效。此外，数据支持我们的观点，乳腺可能是起源的邓宁肿瘤，虽然从球尿道或腮腺的起源不能严格排除。
SummaryThe Dunning tumor, originally described as a carcinoma of the rat dorsal prostate, has for long been used as an experimental model of prostatic cancer. We have recently presented a number of morphological findings that are incompatible with the prostatic origin of the H-subline of the Dunning tumor. In this paper, biochemical and immunohistochemical markers of rat prostate and mammary gland are studied in the R-3327 Dunning H tumor. Pieces of the H tumor were inoculated in male or lactating female rats. The electrophoretic protein pattern of Dunning tumor extracts was more similar to that of the mammary gland than the dorsolateral prostate. Proteins selectively appearing after metabolic labeling in Dunning tumors grown in lactating rats corresponded to labeled proteins in mammary glands from the same animals. Secretory proteins typical of the lateral prostate (SVS II) and dorsal prostate (transglutaminase) could not be detected immunohistochemically in the Dunning tumor. Western blot studies of tumor extracts and slot blot analysis of RNA preparations from the tumor confirmed the absence of SVS II and prostate specific transglutaminase from the Dunning tumor. On the other hand, the presence of mammary gland proteins such as milk fat globule membrane proteins, lactoperoxidase and lactalbumin were detected in the Dunning tumor by immunohistochemistry and Western blotting, but were absent from the dorsolateral prostate. Transferrin-mRNA, expressed in the male urogenital tract and also in the liver and other tissues, was detected in the mammary gland and Dunning tumor, but not in the dorsolateral prostate. The absence of mammary gland secretory β-casein in the Dunning tumor was related to the elevated Ha-ras oncogene expression in the tumor, previously reported to suppress casein expression. The findings clearly demonstrate that the prostate cannot be the origin of the Dunning tumor, presently being used in prostatic cancer research. The designation prostatic adenocarcinoma for this tumor is therefore invalid. Furthermore, the data support our view that mammary gland might be the origin of the Dunning tumor, although the derivation from the bulbourethral or the parotid glands cannot strictly be excluded.
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DOI：
--
发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
White,BA;Bancroft,FC
通讯作者：
Bancroft,FC
与 Dunning R-3327 大鼠前列腺腺癌系统进展相关的染色体变化。
影响因子：
11.2
作者：
Wake,N;Isaacs,J;Sandberg,AA
通讯作者：
Sandberg,AA
Dunning R3327 大鼠前列腺腺癌系统的肌动蛋白丝组织：与转移潜能的相关性。
影响因子：
11.2
作者：
Zachary,JM;Cleveland,G;Kwock,L;Lawrence,T;Weissman,RM;Nabell,L;Fried,FA;Staab,EV;Risinger,MA;Lin,S
通讯作者：
Lin,S
不依赖雄激素的 Dunning R-3327-G 大鼠前列腺腺癌的异常雄激素敏感性：雄激素对肿瘤细胞损失的影响。
影响因子：
11.2
作者：
Humphries,JE;Isaacs,JT
通讯作者：
Isaacs,JT
一种乳腺癌抑制基因及其在大鼠中的作用位点。
影响因子：
11.2
作者：
Isaacs,JT
通讯作者：
Isaacs,JT